p62Dok Antibody

• 产品编号：CST-3912S      品牌：CST       原厂货号：3912S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

p62Dok兔多抗能检测转染的p62Dok蛋白水平。该抗体不与相关蛋白发生交叉反应。该多克隆抗体是通过用与人p62Dok蛋白酪氨酸（398位点）周围残基相一致的合成肽段免疫动物后获得的。该抗体经蛋白A和肽亲和层析纯化。p62Dok (Dok-1)是细胞内一种大部分酪氨酸被磷酸化、GAP相关的、60 kDa蛋白，经不同的酪氨酸激酶作用后会改变结构(1)。p62Dok蛋白包含一个胺端结构域，它可能参与磷脂的相互作用和膜上定位。对该结构域的主要推测是含磷酪氨酸结合区域能与酪氨酸磷酸化蛋白相互作用。该蛋白的羧端区域有许多酪氨酸，它们都是酪氨酸激酶的潜在目标。若这些酪氨酸被磷酸化，它们就能作为含有SH2结构域的蛋白“停泊位点”(2)。p62Dok的过度表达会抑制人胚胎肾293、B细胞中Ras蛋白的活性以及不成熟B细胞系中B细胞抗原受体调节的c-Fos启动子的活化(3)，暗示了p62Dok蛋白可能对Ras信号传递十分重要。此外，p62Dok蛋白的过度表达也许会抑制胰岛素刺激的Akt蛋白活化(4)。

1. Yamanashi, Y. and Baltimore, D. (1997) Cell 88, 205-211.
2. Grimm, J. et al. (2001) J. Cell Biol. 154, 345-354.
3. Yoshida, K. et al. (2000) J. Biol. Chem. 275, 24945-24952.
4. Wick, M. J. et al. (2001) J. Biol. Chem. 276, 42843-42850.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

p62Dok Antibody detects transfected levels of p62Dok proteins. The antibody does not cross-react with related proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr398 of human p62Dok. Antibodies are purified by protein A and peptide affinity chromatography.

Background

p62Dok (Dok-1) is a major tyrosine-phosphorylated, GAP-associated, 60 kDa protein present within the cells transformed by different tyrosine kinases (1). p62Dok contains an amino-terminal pleckstrin homology domain potentially involved in phospholipid interaction and membrane targeting, a central putative phospho-tyrosine binding domain for interacting with tyrosine-phosphorylated proteins. There are numerous tyrosines in its carboxy-terminal region that are potential targets for tyrosine kinases. If phosphorylated, these tyrosines could serve as docking sites for proteins that contain an SH2 domain (2). Overexpression of p62Dok has been shown to inhibit Ras activity in human embryonic kidney 293 cells and B cell antigen receptor-mediated c-Fos promoter activation in an immature B cell line (3), suggesting that p62Dok may play a negative role in Ras signaling. Moreover, p62Dok overexpression may also inhibit insulin-stimulated Akt activation (4).

1. Yamanashi, Y. and Baltimore, D. (1997) Cell 88, 205-211.
2. Grimm, J. et al. (2001) J. Cell Biol. 154, 345-354.
3. Yoshida, K. et al. (2000) J. Biol. Chem. 275, 24945-24952.
4. Wick, M. J. et al. (2001) J. Biol. Chem. 276, 42843-42850.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.