NuMA Antibody

• 产品编号：CST-3888S      品牌：CST       原厂货号：3888S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NuMA Antibody能够检测内源性NuMA总蛋白。该多克隆抗体是由合成的人源的针对NuMA蛋白羧基末端肽段免疫动物生产的。核有丝分裂器蛋白(NuMA)是一种参与有丝分裂纺锤体形成和维持的卷曲蛋白。NuMA在分裂间期的染色质组织过程起其重要作用，从而影响乳腺细胞分化(1,2)。凋亡过程中，NuMA的羧基末端清除可以放大细胞死亡信号通路。NuMA含有多个磷酸化位点，ATM/ATR依赖方式发生的丝氨酸395位磷酸化能够响应DNA损伤(3,4)。

1. Abad, P.C. et al. (2007) Mol Biol Cell 18, 348-61.
2. Lin, H.H. et al. (2007) J Biomed Sci 14, 681-94.
3. Stokes, M.P. et al. (2007) Proc Natl Acad Sci USA 104, 19855-60.
4. Matsuoka, S. et al. (2007) Science 316, 1160-6.
5. Kotak, S. et al. (2013) EMBO J 32, 2517-29.
6. Seldin, L. et al. (2013) Mol Biol Cell 24, 3651-62.

原厂资料：

Specificity / Sensitivity

NuMA Antibody detects endogenous levels of total NuMA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NuMA. 

Background

The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4). 

1. Abad, P.C. et al. (2007) Mol Biol Cell 18, 348-61.
2. Lin, H.H. et al. (2007) J Biomed Sci 14, 681-94.
3. Stokes, M.P. et al. (2007) Proc Natl Acad Sci USA 104, 19855-60.
4. Matsuoka, S. et al. (2007) Science 316, 1160-6.
5. Kotak, S. et al. (2013) EMBO J 32, 2517-29.
6. Seldin, L. et al. (2013) Mol Biol Cell 24, 3651-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.