ERCC1 Antibody

• 产品编号：CST-3885S      品牌：CST       原厂货号：3885S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ERCC1 Antibody能够检测内源性ERCC1总蛋白。该多克隆抗体是由合成的人源的针对ERCC1蛋白的羧基末端肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。DNA修复系统在所有的活细胞中运行管理各种DNA损伤。核苷酸切除修复(NER)在一些比较大的螺旋扭曲损伤的情况下被执行，这些损伤通常由紫外和化学物质引起(1)。切除修复交叉互补基因1(ERCC1)与XPF(充当损伤切除时的5’核酸内切酶)组成复合物(2)。ERCC1-XPF也参与DNA链间交联(ICLs) (3)修复和双链断裂修复(4)。在几种人类肿瘤中包括非小细胞肺癌发现ERCC1的表达和存活率相关并对化学治疗药物有反应(5,6)。

1. Shuck, S.C. et al. (2008) Cell Res 18, 64-72.
2. McDaniel, L.D. and Schultz, R.A. (2008) Adv Exp Med Biol 637, 65-82.
3. Niedernhofer, L.J. et al. (2004) Mol Cell Biol 24, 5776-87.
4. Ahmad, A. et al. (2008) Mol Cell Biol 28, 5082-92.
5. Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
6. Gossage, L. and Madhusudan, S. (2007) Cancer Treat Rev 33, 565-77.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

ERCC1 Antibody recognizes endogenous levels of total ERCC1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human ERCC1. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions such as those brought about by UV and certain chemicals occur (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with XPF, which acts as the 5’ endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). 
 Expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (5,6). 

1. Shuck, S.C. et al. (2008) Cell Res 18, 64-72.
2. McDaniel, L.D. and Schultz, R.A. (2008) Adv Exp Med Biol 637, 65-82.
3. Niedernhofer, L.J. et al. (2004) Mol Cell Biol 24, 5776-87.
4. Ahmad, A. et al. (2008) Mol Cell Biol 28, 5082-92.
5. Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
6. Gossage, L. and Madhusudan, S. (2007) Cancer Treat Rev 33, 565-77.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.