MOB1 Antibody

• 产品编号：CST-3863S      品牌：CST       原厂货号：3863S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MOB1抗体识别内源性的MOB1总蛋白(MOB1α 和 MOB1β).多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段邻近人源MOB1蛋白的Pro15氨基酸残基序列一致。抗体由肽段由蛋白A和亲和层析技术纯化 .MOB1最早被鉴定为酵母中的一种结合Mps的蛋白，对有丝分裂完成和维持染色体整倍体起必要作用（1）。它在果蝇和人中的同源体分别是Mats和MOB1，涉及Hippo信号通路，对控制器官大小，稳态和肿瘤发展起关键作用（2-5）。MOB1结合核内Dbf2相关激酶如LATS1 和2以及 NDR1 和2，并刺激这些激酶活性（6-8）。这种结合被MOB1的苏氨酸磷酸化所促进，主要由MST1 或 MST2对MOB1的苏氨酸磷酸化（9-10）。人类的MOB1蛋白中中，中包含了MOB1α 和MOB1β两种蛋白，分别被96.3%同源的不同基因编码（10）.

1. Luca, F.C. and Winey, M. (1998) Mol Biol Cell 9, 29-46.
2. Edgar, B.A. (2006) Cell 124, 267-73.
3. Saucedo, L.J. and Edgar, B.A. (2007) Nat Rev Mol Cell Biol 8, 613-21.
4. Harvey, K. and Tapon, N. (2007) Nat Rev Cancer 7, 182-91.
5. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
6. Praskova, M. et al. (2008) Curr Biol 18, 311-21.
7. Devroe, E. et al. (2004) J Biol Chem 279, 24444-51.
8. Hergovich, A. et al. (2005) Mol Cell Biol 25, 8259-72.
9. Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
10. Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.

原厂资料：

Specificity / Sensitivity

MOB1 Antibody detects endogenous levels of total MOB1 protein (MOB1α and MOB1β).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro15 of human MOB1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy (1). Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo Signaling Pathway, which has critical roles in controlling organ size, homeostasis and cancer development (2-5). MOB1 binds to members of the nuclear Dbf2-related kinases, such as LATS1 and 2, and NDR1 and 2, and stimulates their kinase activity (6-8). This binding is promoted by the phosphorylation of MOB1 at several Threonine residues by MST1 and/or MST2 (9,10). There are two MOB1 proteins in humans, MOB1α and MOB1β that are encoded by two different genes but have 96.3% identity (10).

1. Luca, F.C. and Winey, M. (1998) Mol Biol Cell 9, 29-46.
2. Edgar, B.A. (2006) Cell 124, 267-73.
3. Saucedo, L.J. and Edgar, B.A. (2007) Nat Rev Mol Cell Biol 8, 613-21.
4. Harvey, K. and Tapon, N. (2007) Nat Rev Cancer 7, 182-91.
5. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
6. Praskova, M. et al. (2008) Curr Biol 18, 311-21.
7. Devroe, E. et al. (2004) J Biol Chem 279, 24444-51.
8. Hergovich, A. et al. (2005) Mol Cell Biol 25, 8259-72.
9. Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
10. Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.