Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody

• 产品编号：CST-3841S      品牌：CST       原厂货号：3841S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody仅检测在threonine 508或505位点磷酸化的转染的LIMK1和LIMK2蛋白。通过人工合成人源LIMK1蛋白Thr508位点周围相应磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。LIM激酶(LIMK1 and LIMK2)是丝氨酸/苏氨酸激酶，该蛋白在它们的氨基端调节区域含有两个锌指结构其被认为是LIM结构(1)。Rho-family GTPases, PAKs和ROCK的下游的LIM激酶涉及肌动蛋白细胞骨架调节(2,3)。PAKs和ROCK激酶使LIMK1或LIMK2蛋白在激活环里保守的Thr508 或Thr505位点磷酸化，这增加了LIMK活性(3-5)。通过在cofilin蛋白的氨基末端Ser3位点残基磷酸化，活化的LIM 激酶抑制cofilin蛋白的肌动蛋白解聚活性 (6,7)。

1. Okano, I. et al. (1995) J. Biol. Chem. 270, 31321-31330.
2. Maekawa, M. et al. (1999) Science 285, 895-898.
3. Edwards, D. C. et al. (1999) Nat. Cell Biol. 1, 253-259.
4. Ohashi, K. et al. (2000) J. Biol. Chem. 275, 3577-3582.
5. Sumi, T. et al. (2001) J. Biol. Chem. 276, 670-676.
6. Arber, S. et al. (1998) Nature 393, 805-809.
7. Yang, N. et al. (1998) Nature 393, 809-812.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody detects transfected levels of LIMK1 and LIMK2 only when phosphorylated at threonine 508 or 505.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr508 of human LIMK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing the LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).

1. Okano, I. et al. (1995) J. Biol. Chem. 270, 31321-31330.
2. Maekawa, M. et al. (1999) Science 285, 895-898.
3. Edwards, D. C. et al. (1999) Nat. Cell Biol. 1, 253-259.
4. Ohashi, K. et al. (2000) J. Biol. Chem. 275, 3577-3582.
5. Sumi, T. et al. (2001) J. Biol. Chem. 276, 670-676.
6. Arber, S. et al. (1998) Nature 393, 805-809.
7. Yang, N. et al. (1998) Nature 393, 809-812.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody