EAAT2 Antibody

• 产品编号：CST-3838S      品牌：CST       原厂货号：3838S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

EAAT2 Antibody 识别内源性的总EAAT2蛋白。该多克隆抗体由合成的人类EAAT2蛋白氨基酸序列对应肽段免疫动物而制成。该抗体使用多肽亲和层析纯化而得。谷氨酸是哺乳动物中枢神经系统主要的兴奋性神经递质。神经传递过程中，谷氨酸被突触前细胞从囊泡释放，突触后细胞上的谷氨酸受体（如NMDA受体，AMPA受体）结合谷氨酸被激活。兴奋性氨基酸转运（EAATs）的调节和维持细胞外谷氨酸浓度低于兴奋水平。此外，谷氨酸转运体可能会限制突触的兴奋时间，这是由共转运三个钠离子和一个质子以及逆转运一个钾离子的电学过程来实现的。五个EAATs（EAAT1-5）的各有特点：EAAT2（GLT-1），主要在星形胶质细胞中表达，但也在视网膜神经元和胎儿发育时表达（1）。EAAT2纯合敲除的小鼠会有自发致命的癫痫发作和急性皮层损伤的倾向（2）。蛋白激酶C在谷氨酸转运时磷酸化EAAT2的Ser113（3）。

1. Amara, S.G. and Fontana, A.C. (2002) Neurochem Int 41, 313-8.
2. Tanaka, K. et al. (1997) Science 276, 1699-702.
3. Casado, M. et al. (1993) J Biol Chem 268, 27313-7.

原厂资料：

Specificity / Sensitivity

EAAT2 Antibody detects endogenous levels of total EAAT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human EAAT2. Antibodies are purified by peptide affinity chromatography.

Background

Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. During neurotransmission, glutamate is released from vesicles of the pre-synaptic cell, and glutamate receptors (e.g. NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing post-synaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels. In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion. Five EAATs (EAAT1-5) are characterized: EAAT2 (GLT-1) is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development (1). Homozygous EAAT2 knockout mice have spontaneous, lethal seizures and an increased predisposition to acute cortical injury (2). PKC phosphorylates Ser113 of EAAT2 and coincides with glutamate transport (3).

1. Amara, S.G. and Fontana, A.C. (2002) Neurochem Int 41, 313-8.
2. Tanaka, K. et al. (1997) Science 276, 1699-702.
3. Casado, M. et al. (1993) J Biol Chem 268, 27313-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.