CIITA Antibody

• 产品编号：CST-3793S      品牌：CST       原厂货号：3793S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CIITA 抗体能够检测转染的CIITA 蛋白总体水平。此多克隆抗体是通过合成人源对应的 CIITA Glu149位点周围的肽段来免疫动物而获得。抗体是通多肽亲和层析纯化。MHC II类 (MHC-II) 蛋白在细胞免疫中发挥了重要的作用，他们的表达主要是通过无DNA结合转录因子 CIITA (MHC II类 反式作用子)来调节(1,2)。CIITA 的表达通过IFN-γ上调并返回来增强MHC-II的表达和抑制胶原质的表达(3,4)。CIITA只有少数的转录靶标, 而且绝大多数参与到MHC介导的抗原表达(5)。CIITA的突变与免疫缺陷遗传病赤裸的淋巴细胞(BLS)综合症有关，它的一个重要的特点是几乎没有MHC-II的表达(也叫做MHC-II缺陷) (6,7)。

1. Ting, J.P. and Trowsdale, J. (2002) Cell 109 Suppl, S21-33.
2. Drozina, G. et al. (2005) Curr Top Microbiol Immunol 290, 147-70.
3. Dong, Y. et al. (1999) J Immunol 162, 4731-9.
4. Butticè, G. et al. (2006) Circ Res 98, 472-9.
5. Krawczyk, M. et al. (2008) PLoS Genet 4, e1000058.
6. Reith, W. and Mach, B. (2001) Annu Rev Immunol 19, 331-73.
7. Waldburger, J.M. et al. (2000) Immunol Rev 178, 148-65.

原厂资料：

Specificity / Sensitivity

CIITA Antibody detects transfected levels of total CIITA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu149 of human CIITA. Antibodies are purified by peptide affinity chromatography.

Background

MHC class II (MHC-II) proteins play critical roles in cellular immune responses and their expression is mainly regulated by the non-DNA binding transcription factor CIITA (MHC class II transactivator) (1,2). CIITA expression is upregulated by IFN-γ and it in turn enchances MHC-II expression and represses collagen expression (3,4). CIITA has a limited number of transcriptional targets, most of which are involved in MHC-mediated antigen presentation (5). Mutations in the CIITA are associated with the hereditary immunodeficiency disease Bare Lymphocyte Syndrome (BLS) which is characterized by a nearly complete absence of MHC-II expression (also referred to as MHC-II deficiency) (6,7).

1. Ting, J.P. and Trowsdale, J. (2002) Cell 109 Suppl, S21-33.
2. Drozina, G. et al. (2005) Curr Top Microbiol Immunol 290, 147-70.
3. Dong, Y. et al. (1999) J Immunol 162, 4731-9.
4. Butticè, G. et al. (2006) Circ Res 98, 472-9.
5. Krawczyk, M. et al. (2008) PLoS Genet 4, e1000058.
6. Reith, W. and Mach, B. (2001) Annu Rev Immunol 19, 331-73.
7. Waldburger, J.M. et al. (2000) Immunol Rev 178, 148-65.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.