Myc-Tag (9B11) Mouse mAb (Alexa Fluor? 555 Conjugate)

产品编号：CST-3756S 品牌：CST 原厂货号：3756S
产品分类：抗体 > 一抗 > 染料标记抗体
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4°C

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描述：

Myc-Tag (9B11) Mouse mAb （Alexa Fluor ® 555标记）可以检测细胞中包含Myc标为标签的蛋白。此抗体在转染后哺乳动物细胞中可以识别Myc-Tag。单克隆抗体是采用与人类c-Myc (EQKLISEEDL)的410-419残基相对应的合成肽段免疫动物而生产的。抗体在最佳条件下与Alexa Fluor ® 555结合，F/P为2-6.此种抗体结合了Alexa Fluor ® 555荧光染料，已经过内部测试，可以直接在转染Myc-标签蛋白的细胞中应用于免疫荧光。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。Myc标为标签在细菌、酵母和哺乳动物细胞系统中广泛应用于检测重组蛋白的表达（1）。

1、Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.

原厂资料：

Specificity / Sensitivity

Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 555 Conjugate) detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.

Description

This Cell Signaling Technology antibody was conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluorescence in cells transfected with Myc-tagged protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).

1、Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.