JunB (P169) Antibody

• 产品编号：CST-3755S      品牌：CST       原厂货号：3755S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

JunB (P169) Antibody 兔多抗能检测内源性总JunB蛋白水平。该单克隆抗体是采用与人源JunB蛋白Pro169位点周围残基相一致的合成肽段免疫动物后获得的。该抗体经肽亲和层析纯化。JunB蛋白是碱性亮氨酸拉链（bZIP）转录因子，属于Jun家族。该家族包括c-Jun 、JunD蛋白。Jun家族成员与Fos 、ATF蛋白形成同源二聚体或异源二聚体，构成功能性转录因子AP-1（活化蛋白1），它的活性由各种生理和病理刺激调节，如生长因子、传染病和应激性信号(1-4)。由于JunB 蛋白有时会拮抗c-Jun的转录活性，因此该蛋白在小鼠发育过程中可能是Jun蛋白的功能性替代物(5-7)。JunB 蛋白能调节造血干细胞数量，并在慢性粒细胞白血病 (CML)和急性髓细胞性白血病 (AML)的发病机理中发挥重要作用(8,9)。

1. Busch, S.J. and Sassone-Corsi, P. (1990) Trends Genet. 6, 36-40.
2. Shaulian, E. and Karin, M. (2002) Nat. Cell Biol. 4, E131-E136.
3. Hess, J. et al. (2004) J. Cell Sci. 117, 5965-5973.
4. Mechta-Grigoriou, F. et al. (2001) Oncogene 20, 2378-2389.
5. Chiu, R. et al. (1989) Cell 59, 979-986.
6. Schütte, J. et al. (1989) Cell 59, 987-997.
7. Passegué, E. et al. (2002) Nat. Genet. 30, 158-166.
8. Steidl, U. et al. (2006) Nat. Genet. 38, 1269-1277.
9. Passegué, E. et al. (2004) Cell 119, 431-443.

原厂资料：

Specificity / Sensitivity

JunB (P169) Antibody detects the endogenous level of total JunB protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro169 of human JunB. Antibodies are purified by peptide affinity chromatography.

Background

JunB is a basic region, leucine zipper (bZIP) transcription factor belonging to the Jun family that includes c-Jun and JunD. Jun family members homodimerize or heterodimerize with Fos and ATF proteins to form a functional transcription factor AP-1 (activator protein 1), whose activity is regulated by a variety of physiological and pathological stimuli such as growth factors, infections, and stress signals (1-4). While JunB sometimes antagonizes c-Jun transcriptional activity, it may functionally substitute for c-Jun during development in mice (5-7). JunB regulates hematopoietic stem cell number and plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) (8,9).

1. Busch, S.J. and Sassone-Corsi, P. (1990) Trends Genet. 6, 36-40.
2. Shaulian, E. and Karin, M. (2002) Nat. Cell Biol. 4, E131-E136.
3. Hess, J. et al. (2004) J. Cell Sci. 117, 5965-5973.
4. Mechta-Grigoriou, F. et al. (2001) Oncogene 20, 2378-2389.
5. Chiu, R. et al. (1989) Cell 59, 979-986.
6. Schütte, J. et al. (1989) Cell 59, 987-997.
7. Passegué, E. et al. (2002) Nat. Genet. 30, 158-166.
8. Steidl, U. et al. (2006) Nat. Genet. 38, 1269-1277.
9. Passegué, E. et al. (2004) Cell 119, 431-443.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.