Phospho-5-Lipoxygenase (Ser271) Antibody

• 产品编号：CST-3748S      品牌：CST       原厂货号：3748S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-5-Lipoxygenase (Ser271) 抗体能够检测转染水平的在Ser271位点磷酸化的5-lipoxygenase蛋白。此多克隆抗体是通过合成人源对应的5-lipoxygenase Ser271位点周围的磷肽段来免疫动物而获得。5-Lipoxygenase (5-LO, ALOX5)是从花生四烯酸生物合成白细胞三烯LTA4过程中一个重要的催化酶(1,2)。 白细胞三烯合成也需要5-lipoxygenase激活蛋白(FLAP, ALOX5AP), 此蛋白系细胞膜结合蛋白，能够结合花生四烯酸并激活5-LO。一些相关的白细胞三烯 (i.e. B4, C4, D4) 起源于LTA4 和一些在免疫反应调节中发挥作用的脂类介质。5-LO主要在中性粒细胞，外周血单核细胞，巨噬细胞，肥大细胞中表达(1,3)。在一些癌细胞中5-LO过表达并与诊断不利有很大的关系(1,4)。根据不同的细胞种类， 5-LO或者定位在细胞质或者是静止细胞的细胞核(5)。受到刺激后, 5-LO 定位到细胞核中并与FLAP相关催化 LTA4的合成(2,3)。磷酸化特定残基能调控5-LO酶活性。通过PKA家族的激酶磷酸化5-LO的Ser523位点抑制氧化酶的活性(6,7)，同时，在体内，MAPKAP2 和ERK家族激酶磷酸化Ser271和Ser663位点能够刺激5-LO酶的活性(8,9)。

1. Woods, J.W. et al. (1995) J Clin Invest 95, 2035-46.
2. Evans, J.F. et al. (2008) Trends Pharmacol Sci 29, 72-8.
3. Radmark, O. et al. (2007) Trends Biochem Sci 32, 332-41.
4. Chen, X. et al. (2006) Curr Cancer Drug Targets 6, 613-22.
5. Werz, O. (2002) Curr Drug Targets Inflamm Allergy 1, 23-44.
6. Luo, M. et al. (2004) J Biol Chem 279, 41512-20.
7. Luo, M. et al. (2005) J Biol Chem 280, 40609-16.
8. Werz, O. et al. (2002) FASEB J 16, 1441-3.
9. Werz, O. et al. (2002) J Biol Chem 277, 14793-800.

原厂资料：

Specificity / Sensitivity

Phospho-5-Lipoxygenase (Ser271) Antibody detects transfected levels of 5-lipoxygenase protein only when phosphorylated at Ser271.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser271 of human 5-lipoxygenase protein.

Background

5-Lipoxygenase (5-LO, ALOX5) is an important catalytic enzyme responsible for the biosynthesis of leukotriene LTA4 from arachidonic acid (1,2). Leukotriene synthesis also requires 5-lipoxygenase-activating protein (FLAP, ALOX5AP), a nuclear membrane-bound protein that binds arachidonic acid and is thought to activate 5-LO. A number of related leukotrienes (i.e. B4, C4, D4) are derived from LTA4 and together these lipid mediators function in immune reaction regulation. 5-LO is primarily expressed in polymorphonuclear leukocytes, peripheral blood monocytes, macrophages, and mast cells (1,3). Overexpression of 5-LO protein is seen in certain cancer cells and is associated with poor diagnosis (1,4). Depending upon the cell type, 5-LO is localized to either the cytosol or the nucleus of quiescent cells (5). Following stimulation, 5-LO translocates to the nucleus and associates with FLAP to catalyze LTA4 synthesis (2,3). Phosphorylation of specific residues can regulate 5-LO enzymatic activity. Phosphorylation of 5-LO at Ser523 by PKA family kinases inhibits oxygenase activity (6,7) while MAPKAP2 and ERK family kinase phosphorylation at Ser271 and Ser663 stimulates 5-LO enzymatic activity in vivo (8,9).

1. Woods, J.W. et al. (1995) J Clin Invest 95, 2035-46.
2. Evans, J.F. et al. (2008) Trends Pharmacol Sci 29, 72-8.
3. Radmark, O. et al. (2007) Trends Biochem Sci 32, 332-41.
4. Chen, X. et al. (2006) Curr Cancer Drug Targets 6, 613-22.
5. Werz, O. (2002) Curr Drug Targets Inflamm Allergy 1, 23-44.
6. Luo, M. et al. (2004) J Biol Chem 279, 41512-20.
7. Luo, M. et al. (2005) J Biol Chem 280, 40609-16.
8. Werz, O. et al. (2002) FASEB J 16, 1441-3.
9. Werz, O. et al. (2002) J Biol Chem 277, 14793-800.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.