SDF1 Antibody

• 产品编号：CST-3740S      品牌：CST       原厂货号：3740S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SDF1 抗体能够检测转染水平的SDF1蛋白，并且可能识别SDF1α 和SDF1β两种形式。此多克隆抗体是通过合成人源对应的SDF1 Gln80位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。基质细胞起源因子1(SDF1, CXCL12)是一个小分子, 促趋化因子通过与其同源的7次跨膜G蛋白偶联受体的相互作用而调控白细胞运输(1)。SDF1的受体, CXCR4, 也作为一个共受体而使人免疫缺陷病毒进入到目标细胞(2)。SDF1可能调控引导并维持CXCR4表达干细胞或者是祖先细胞, 包括胚胎细胞核很多成体干细胞(3,4)。很多的癌细胞表达CXCR4, 表明SDF1在癌细胞的转移中发挥作用(5,6)。可变性剪切和在成熟过程中的分化产生了一对SDF1不同形式 (SDF1α 和SDF1β)具有不同的性质和生物活性(7)。另外的SDF1的不同形式也有报道(8,9)。

1. Maekawa, T. and Ishii, T. (2000) Intern. Med. 39, 90-100.
2. Ray, N. and Doms, R.W. (2006) Curr. Top. Microbiol. Immunol. 303, 97-120.
3. Sugiyama, T. et al. (2006) Immunity 25, 977-988.
4. Ara, T. et al. (2003) Immunity 19, 257-267.
5. Kucia, M. et al. (2004) J. Mol. Histol. 35, 233-245.
6. Ratajczak, M.Z. et al. (2006) Leukemia 20, 1915-1924.
7. De La Luz Sierra, M. et al. (2004) Blood 103, 2452-2459.
8. Gleichmann, M. et al. (2000) Eur. J. Neurosci. 12, 1857-1866.
9. Yu, L. et al. (2006) Gene 374, 174-179.

原厂资料：

Specificity / Sensitivity

SDF1 Antibody detects recombinant SDF1 protein and is expected to recognize both SDF1α and SDF1β isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln80 of human SDF1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The stromal cell derived factor 1 (SDF1, CXCL12) is a small, pro-inflammatory chemoattractant cytokine that regulates leukocyte trafficking through interactions with its cognate 7-transmembrane G protein-coupled receptors (1). The SDF1 receptor, CXCR4, also serves as a coreceptor for the entry of human immunodeficiency virus into target cells (2). SDF1 may regulate homing and maintenance of CXCR4-expressing stem or progenitor cells, including embryonic and many somatic stem cells (3,4). Many cancer cells express CXCR4, suggesting that SDF1 plays a role in cancer metastasis (5,6). Alternative splicing and differential processing during maturation produce a pair of SDF1 isoforms (SDF1α and SDF1β) that have different properties and biological activities (7). Additional isoforms of SDF1 have been reported (8,9).

1. Maekawa, T. and Ishii, T. (2000) Intern. Med. 39, 90-100.
2. Ray, N. and Doms, R.W. (2006) Curr. Top. Microbiol. Immunol. 303, 97-120.
3. Sugiyama, T. et al. (2006) Immunity 25, 977-988.
4. Ara, T. et al. (2003) Immunity 19, 257-267.
5. Kucia, M. et al. (2004) J. Mol. Histol. 35, 233-245.
6. Ratajczak, M.Z. et al. (2006) Leukemia 20, 1915-1924.
7. De La Luz Sierra, M. et al. (2004) Blood 103, 2452-2459.
8. Gleichmann, M. et al. (2000) Eur. J. Neurosci. 12, 1857-1866.
9. Yu, L. et al. (2006) Gene 374, 174-179.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.