Caspr2 Antibody

• 产品编号：CST-3731S      品牌：CST       原厂货号：3731S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Caspr2 Antibody 兔多抗识别内源性的总Caspr2蛋白。该多克隆抗体由合成的人类Caspr2蛋白中Glu1320附近肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。Contactin相关蛋白2（Caspr2）是I型跨膜蛋白和neurexin超家族成员，通过Neurexin IV-Caspr-Paranodin（NCP）复合物介导中枢神经系统细胞与细胞相互作用（1）。一种由TAG-1、Caspr2、K+ 通道、PSD95和蛋白质4.1B构成的多蛋白复合物介导有髓轴突juxtaparanodal区域的分子相互作用，同种TAG-1相互作用介导这种复合物与胶质细胞的结合（2,3）。Caspr2蛋白质定位于有髓突触的juxtaparanodal区域，其与免疫球蛋白样细胞粘和分子TAG-1形成一个顺式复合物。Caspr2也能和Shaker钾通道Kv1.1、Kv1.2和它们的Kvβ2亚基结合。Caspr2羧基末端的PDZ结构域接到Caspr2-钾通道结合。钾通道的正确定位需要Caspr2，当Caspr2缺失时通道在节间重新分布（1-3）。此外，Caspr2结合4.1B蛋白，将蛋白复合物连接到轴突细胞骨架（4）。局灶性癫痫、皮质发育不良和抽动秽语综合征与在Caspr2基因突变有关（5,6）。

1. Poliak, S. et al. (1999) Neuron 24, 1037-47.
2. Poliak, S. et al. (2003) J Cell Biol 162, 1149-60.
3. Traka, M. et al. (2003) J Cell Biol 162, 1161-72.
4. Denisenko-Nehrbass, N. et al. (2003) Eur J Neurosci 17, 411-6.
5. Verkerk, A.J. et al. (2003) Genomics 82, 1-9.
6. Strauss, K.A. et al. (2006) N Engl J Med 354, 1370-7.

原厂资料：

Specificity / Sensitivity

Caspr2 Antibody detects endogenous levels of total Caspr2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide sourrounding Glu1320 of human Caspr2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Contactin-associated protein 2 (Caspr2) is a type I transmembrane protein and member of the neurexin superfamily that mediates nervous system cell-cell interactions through the Neurexin IV-Caspr-Paranodin (NCP) complex (1). A multiprotein complex consisting of TAG-1, Caspr2, K+ channel, PSD95 and protein 4.1B mediates the molecular interactions at the juxtaparanodal region of myelinated axons, with homophilic TAG-1 interactions mediating the binding of this complex to glia (2,3). 
 Caspr2 protein localizes to juxtaparanodal regions of myelinated axons where it forms a cis-complex with the immunoglobulin-like cell adhesion molecule TAG-1. Caspr2 also binds to Shaker K+ channels Kv1.1, Kv1.2, and their Kvβ2 subunit. A PDZ domain at the Caspr2 carboxy terminus mediates the Caspr2-K+ channel association. Caspr2 is required for proper K+ channel localization, as Caspr2 deletion causes the redistribution of channels along the internodes (1-3). Furthermore, Caspr2 binds to protein 4.1B and connects the protein complex to the axonal cytoskeleton (4). Mutations in the Caspr2 gene have been linked to focal epilepsy, cortical dysplasia and Gilles de la Tourette syndrome (5,6).

1. Poliak, S. et al. (1999) Neuron 24, 1037-47.
2. Poliak, S. et al. (2003) J Cell Biol 162, 1149-60.
3. Traka, M. et al. (2003) J Cell Biol 162, 1161-72.
4. Denisenko-Nehrbass, N. et al. (2003) Eur J Neurosci 17, 411-6.
5. Verkerk, A.J. et al. (2003) Genomics 82, 1-9.
6. Strauss, K.A. et al. (2006) N Engl J Med 354, 1370-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.