OSR1 Antibody

• 产品编号：CST-3729S      品牌：CST       原厂货号：3729S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

OSR1 Antibody检测内源性OSR1总蛋白。该抗体不能与SPAK蛋白或GCK家族的其它成员发生相互作用。通过人工合成人源OSR1蛋白氨基末端周围相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化蛋白。SPAK (STE20/SPS1-related Pro/Ala-rich kinase)和OSR1 (oxidative stress responsive 1)是GCK丝氨酸/苏氨酸激酶家族成员。过表达和体外研究证明SPAK蛋白能够激活p38 MAP激酶，其表明在应激反应下SPAK蛋白的一个可能的角色(1)。酵母双杂交试验揭示SPAK和OSR1蛋白结合到Na-K-2Cl转运蛋白NKCC1和NKCC2以及K-Cl转运蛋白KCC3 (2)。WNK1和WNK4激酶使SPAK蛋白在Thr243/247和Ser380位点发生磷酸化(3-5)。同样地，WNK1和WNK4激酶使OSR1蛋白在Thr185和Ser315位点发生磷酸化(3, 4)。在这些位点的磷酸化刺激SPAK和OSR1的激活，这导致NKCC1的磷酸化和提高NKCC1的活性(3-5)。在T细胞的激活反应下，通过PKCθ激酶使SPAK蛋白也在Ser311位点磷酸化。Ser311被Ala替换或SPAK蛋白特异siRNA干扰显著性降低TCR/CD28诱导AP-1激活，这认为SPAK也涉及T细胞信号(6)。

1. Johnston, A.M. et al. (2000) Oncogene 19, 4290-7.
2. Piechotta, K. et al. (2002) J Biol Chem 277, 50812-9.
3. Vitari, A.C. et al. (2005) Biochem J 391, 17-24.
4. Moriguchi, T. et al. (2005) J Biol Chem 280, 42685-93.
5. Gagnon, K.B. et al. (2006) Mol Cell Biol 26, 689-98.
6. Li, Y. et al. (2004) EMBO J 23, 1112-22.

原厂资料：

Specificity / Sensitivity

OSR1 Antibody detects endogenous levels of total OSR1 protein. This antibody does not cross-react with SPAK or other members of the GCK family.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human OSR1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family serine/threonine kinases. Overexpression and in vitro studies demonstrate that SPAK is able to activate p38 MAP kinase indicating a possible role for SPAK in the stress response (1). Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3 (2). WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380 (3-5). Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315 (3, 4). Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity (3-5). SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knock-down of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well (6).

1. Johnston, A.M. et al. (2000) Oncogene 19, 4290-7.
2. Piechotta, K. et al. (2002) J Biol Chem 277, 50812-9.
3. Vitari, A.C. et al. (2005) Biochem J 391, 17-24.
4. Moriguchi, T. et al. (2005) J Biol Chem 280, 42685-93.
5. Gagnon, K.B. et al. (2006) Mol Cell Biol 26, 689-98.
6. Li, Y. et al. (2004) EMBO J 23, 1112-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.