TNF-R2 Antibody

• 产品编号：CST-3727S      品牌：CST       原厂货号：3727S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TNF-R2 Antibody 能够检测内源TNF-R2总蛋白。该多克隆抗体是由合成的人源TNF-R2蛋白胞内区Asp335处相应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。TNF-α是一个重要的细胞因子，它由很多种细胞产生，包括中性粒细胞、激活的淋巴细胞、巨噬细胞和NK细胞。它在炎症反应和凋亡过程中发挥重要的作用(1)。TNF-α 存在细胞膜锚定和可溶性形式，这两种形式都有生物活性。 两个受体介导对TNF-α的响应，一个是广泛表达的TNF-R1，另一个是主要在免疫和内皮细胞表达的TNF-R2(2)。 TNF-α的拮抗剂已经被证实是风湿性关节炎和免疫紊乱的治疗靶标(3)。TNF-α的两个受体, TNF-R1 (55 kDa) 和 TNF-R2 (75 kDa)， 能够介导不同的细胞应答(4,5)。据报道，很多时候TNF引导的细胞毒性是通过TNF-R1(6,7)。与此相反，TNF-R2对于T细胞信号通路和感染应答十分重要（7,8）。 TNF-R2结合TRAF家族的不同成员导致NF-κB的激活（9,10）。这两个可溶性的受体都已经被鉴定，他们能够结合TNF-α，并在免疫紊乱过程中发挥了重要的作用(11,12)。

1. Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56.
2. Locksley, R.M. et al. (2001) Cell 104, 487-501.
3. Taylor, P.C. et al. (2004) Curr Opin Biotechnol 15, 557-63.
4. Tartaglia, L.A. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 9292-9296.
5. Peschon, J.J. et al. (1998) J. Immunol. 160, 943-952.
6. Tartaglia, L.A. et al. (1993) Cell 73, 213-216.
7. Rothe, J. et al. (1993) Nature 364, 798-802.
8. Aspalter, R.M. et al. (2003) J. Leukoc. Biol. 74, 572-582.
9. Rothe, M. et al. (1994) Cell 78, 681-692.
10. Rothe, M. et al. (1995) Science 269, 1424-1427.
11. Humbert, M. et al. (1994) Am. J. Respir. Crit. Care Med. 149, 1681-1685.
12. Schröder, J. et al. (1995) Infection 23, 143-8.

原厂资料：

Specificity / Sensitivity

TNF-R2 Antibody detects endogenous levels of total TNF-R2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp335 within the intracellular region of human TNF-R2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

TNF-α is an important cytokine produced by numerous cell types including neutrophils, activated lymphoctyes, macrophages an NK cells. It plays a critical role in inflammatory responses and in apoptosis (1). TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3). The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa) can mediate distinct cellular responses (4,5). In most cases cytotoxicity elicited by TNF has been reported to act throught TNF-R1 (6,7). In contrast, TNF-R2 appears to be important in T cell signaling and responses to infection (7,8). TNF-R2 binds to distinct members of the TRAF family leading to the activation of NF-κB (9,10). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders (11,12).

1. Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56.
2. Locksley, R.M. et al. (2001) Cell 104, 487-501.
3. Taylor, P.C. et al. (2004) Curr Opin Biotechnol 15, 557-63.
4. Tartaglia, L.A. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 9292-9296.
5. Peschon, J.J. et al. (1998) J. Immunol. 160, 943-952.
6. Tartaglia, L.A. et al. (1993) Cell 73, 213-216.
7. Rothe, J. et al. (1993) Nature 364, 798-802.
8. Aspalter, R.M. et al. (2003) J. Leukoc. Biol. 74, 572-582.
9. Rothe, M. et al. (1994) Cell 78, 681-692.
10. Rothe, M. et al. (1995) Science 269, 1424-1427.
11. Humbert, M. et al. (1994) Am. J. Respir. Crit. Care Med. 149, 1681-1685.
12. Schröder, J. et al. (1995) Infection 23, 143-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.