Pin1 Antibody

• 产品编号：CST-3722S      品牌：CST       原厂货号：3722S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Pin1 Antibody能够检测内源性Pin1总蛋白。该抗体不与其他蛋白发生交叉反应。该多克隆抗体是由合成的人源的针对Pin1蛋白羧基末端肽段多肽免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。 Pin1是小细胞蛋白肽脯氨酰异构酶（PPIase）家庭成员，参与哺乳动物细胞周期的G2/M期转换(1-6)。Pin1是一个18 kDa的小蛋白，含有两个独立的功能结构域：一个氨基末端WW结构域和一个羧基末端Pplase结构域。Pin1能够与几个有丝分裂磷蛋白相互作用，包括Plk1、cdc25C和cdc27，而且被认为能够充当这些靶分子的磷酸化依赖性Pplase(7-9)。

1. Lu, P. J. et al. (1999) Science 283, 1325-1328.
2. Verdecia, M. A. et al. (2000) Nat. Struct. Biol. 7, 639-643.
3. Lu, K. P. et al. (1996) Nature 380, 544-547.
4. Zhou, X. Z. et al. (2000) Mol. Cell 6, 873-883.
5. Wu, X. et al. (2000) EMBO J. 19, 3727-3738.
6. Winkler, K. E. et al. (2000) Science 287, 1644-1647.
7. Crenshaw, D. G. et al. (1998) EMBO J. 17, 1315-1327.
8. Shen, M. et al. (1998) Genes Dev. 12, 706-720.
9. Yaffe, M. B. et al. (1997) Science 278, 1957-1960.

原厂资料：

Specificity / Sensitivity

Pin1 Antibody detects endogenous levels of total Pin1 protein. The antibody does not cross-react with other proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human Pin1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Pin1, a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2/M transition of the mammalian cell cycle (1-6). Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).

1. Lu, P. J. et al. (1999) Science 283, 1325-1328.
2. Verdecia, M. A. et al. (2000) Nat. Struct. Biol. 7, 639-643.
3. Lu, K. P. et al. (1996) Nature 380, 544-547.
4. Zhou, X. Z. et al. (2000) Mol. Cell 6, 873-883.
5. Wu, X. et al. (2000) EMBO J. 19, 3727-3738.
6. Winkler, K. E. et al. (2000) Science 287, 1644-1647.
7. Crenshaw, D. G. et al. (1998) EMBO J. 17, 1315-1327.
8. Shen, M. et al. (1998) Genes Dev. 12, 706-720.
9. Yaffe, M. B. et al. (1997) Science 278, 1957-1960.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.