p27 Kip1 (D69C12) XP? Rabbit mAb

• 产品编号：CST-3686S      品牌：CST       原厂货号：3686S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

p27 Kip1 (D69C12) XP® Rabbit mAb 能够检测内源性p27 Kip1总蛋白。该单克隆抗体是由合成的人源的针对p27 Kip1蛋白氨基末端的肽段免疫动物生产的。p27 Kip1是周期蛋白依赖性激酶抑制剂Cip/Kip家族的一员。同其类似物一样，如p57 Kip2 和p21 Waf1/Cip1，其巩固G1限制点的能力源于CDK2/cyclin E和其它CDK/cyclin复合体的结合抑制。在静止期细胞和cAMP或其它细胞周期阴性调节剂处理的细胞中，p27的表达是上调的。白细胞介素2或者其它有丝分裂原能够诱导p27的下调；该过程涉及p27的磷酸化和泛素化-蛋白酶体途径介导的降解(1-4)。

1. Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23.
2. Polyak, K. et al. (1994) Genes Dev 8, 9-22.
3. Kato, J.Y. et al. (1994) Cell 79, 487-96.
4. Vlach, J. et al. (1997) EMBO J 16, 5334-44.

原厂资料：

Specificity / Sensitivity

p27 Kip1 (D69C12) XP® Rabbit mAb detects endogenous levels of total p27 Kip1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human p27 Kip1.

Background

p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin 2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).

1. Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23.
2. Polyak, K. et al. (1994) Genes Dev 8, 9-22.
3. Kato, J.Y. et al. (1994) Cell 79, 487-96.
4. Vlach, J. et al. (1997) EMBO J 16, 5334-44.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.