TCEB3/Elongin A Antibody

• 产品编号：CST-3685S      品牌：CST       原厂货号：3685S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TCEB3/Elongin A Antibody能够检测内源性TCEB3/Elongin A总蛋白水平。通过合成的人源TCEB3/Elongin A蛋白羧基端相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。Elongin复合物是一个异源三聚体，它是由TCEB3/Elongin A、Elongin B (TCEB2)和Elongin C (TCEB1)亚基组成(1-3)。Elongin复合物调节RNA polymerase II (RNAPII)转录延伸率，这是通过在沿着DNA多位点上释放瞬间暂停。TCEB3/Elongin A是转录激活亚基，而Elongin B和C亚基起到一个调节作用(3,4)。TCEB3/Elongin A可能对于一些细胞周期调节基因的表达是需要的，并且缺少TCEB3/Elongin A蛋白的胚胎干细胞 (embryonic stem,ES)显示在细胞大小、生长和细胞周期分布上不正常(5)。此外，研究显示Elongin复合物与cullin蛋白家族和UV诱导的DNA损伤下RING finger蛋白Cul5/Rbx2相互作用，这通过一个E3泛素连接酶的部分泛素化和降解从而移除了在DNA损伤位点上阻遏的RNAPII蛋白(6)。

1. Bradsher, J.N. et al. (1993) J Biol Chem 268, 25587-93.
2. Garrett, K.P. et al. (1994) Proc Natl Acad Sci U S A 91, 5237-41.
3. Aso, T. et al. (1995) Science 269, 1439-43.
4. Aso, T. et al. (1996) EMBO J 15, 5557-66.
5. Yamazaki, K. et al. (2003) J Biol Chem 278, 13585-9.
6. Yasukawa, T. et al. (2008) EMBO J 27, 3256-66.

原厂资料：

Specificity / Sensitivity

TCEB3/Elongin A Antibody recognizes endogenous levels of total TCEB3/Elongin A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TCEB3/Elongin A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Elongin complex is a heterotrimer composed of TCEB3/Elongin A, Elongin B (TCEB2), and Elongin C (TCEB1) subunits (1-3). The Elongin complex regulates the rate of RNA polymerase II (RNAPII) transcription elongation by releasing the transient pausing of RNAPII at multiple sites along the DNA. TCEB3/Elongin A is the transcriptionally active subunit, while Elongin B and C subunits play a regulatory role (3,4). TCEB3/Elongin A may be required for expression of a subset of cell cycle regulated genes, and embryonic stem (ES) cells lacking TCEB3/Elongin A show abnormalities in cell size, growth, and cell cycle distribution (5). In addition, the Elongin complex has been shown to interact with the cullin family and RING finger proteins Cul5/Rbx2 upon UV-induced DNA damage, removing arrested RNAPII at sites of DNA damage by ubiquitination and degradation as part of an E3 ubiquitin ligase (6).

1. Bradsher, J.N. et al. (1993) J Biol Chem 268, 25587-93.
2. Garrett, K.P. et al. (1994) Proc Natl Acad Sci U S A 91, 5237-41.
3. Aso, T. et al. (1995) Science 269, 1439-43.
4. Aso, T. et al. (1996) EMBO J 15, 5557-66.
5. Yamazaki, K. et al. (2003) J Biol Chem 278, 13585-9.
6. Yasukawa, T. et al. (2008) EMBO J 27, 3256-66.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.