GAPDH (14C10) Rabbit mAb (HRP Conjugate)

• 产品编号：CST-3683S      品牌：CST       原厂货号：3683S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

GAPDH (14C10) Rabbit mAb 兔单抗(HRP Conjugate)用于检测内源性GAPDH总蛋白水平。单克隆抗体通过用对应于人源GAPDH蛋白C端序列的合成肽免疫动物制备而得，抗体通过其氨基与辣根过氧物酶的糖基共价连接。抗体通过其氨基与辣根过氧物酶的糖基共价连接，非交连的GAPDH (14C10) Rabbit mAb 兔单抗#2118可以识别人，小鼠，大鼠和猴子的GAPDH。CST认为GAPDH (14C10) (HRP conjugate) #3683同时也能检测这些物种的GAPDH。甘油醛－3－磷酸脱氢酶（GAPDH）在糖酵解过程中将3-磷酸甘油醛进一步磷酸化。虽然在不同组织中的表达水平有差异，但GAPDH普遍被认为是一个组成型表达的持家蛋白。正是由于这个原因 ，GAPDH的mRNA和蛋白通常在对其它有变化的靶蛋白测定实验时作为对照。最近的研究阐明了GAPDH在细胞凋亡（2），基因表达（3）和核转运（4）过程中的作用。GAPDH还可能在神经退行性 疾病如亨廷顿和阿尔茨海默病的发生起一定作用（4,5）。

1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3. Zheng, L. et al. (2003) Cell 114, 255-66.
4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

原厂资料：

Specificity / Sensitivity

GAPDH (14C10) Rabbit mAb (HRP Conjugate) detects endogenous levels of total GAPDH protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH. The antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The unconjugated GAPDH (14C10) Rabbit mAb #2118 reacts with human, mouse, rat and monkey GAPDH. CST expects that GAPDH (14C10) (HRP conjugate) #3683 will also recognize GAPDH in these species.

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3) and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).

1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3. Zheng, L. et al. (2003) Cell 114, 255-66.
4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.