MSK2 (D41A4) XP? Rabbit mAb

• 产品编号：CST-3679P      品牌：Cell Signaling Technology       原厂货号：3679P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MSK2 (D41A4) XP® Rabbit mAb兔单抗能检测内源性总MSK2蛋白水平。该单克隆抗体通过使用与人源MSK2蛋白Pro751位点周围区域相一致的合成肽段免疫动物而获得。有丝分裂原和应激活化的蛋白激酶1（MSK1）和MSK2都是丝氨酸/苏氨酸激酶，它们能促进应激或有丝分裂诱导的细胞(1-4,7, 8)中以及LPS刺激的巨噬细胞中的即早基因的快速转录(9)。MSK2也被称为RSKB，包含两个催化结构域，已被证明能够直接与p38 MAPK发生反应(10)。在HeLa细胞和鼠科动物胚胎成纤维细胞(MEFs)中，MSK2能够在肿瘤坏死因子、上皮生长因子或佛波酯的刺激下，以一种p38、ERK依赖型方式被磷酸化进而活化(8,11)。两个催化区域的活化环内Ser196位和Thr568位点的磷酸化是全激酶活化所必须的(11)。MSK1、MSK2都包含一个功能性细胞核定位序列，这是核靶向所必须的(10)。与它们的核定位一致，这些激酶能够调节应激和有丝分裂原应答的转录效应。在HeLa细胞、MEFs中，MSK2的活化导致了组蛋白H3、HMG-14、CREB和ATF14的快速磷酸化以及组蛋白H3的乙酰化作用，这些都是与即早基因的转录相关。(3,4,6,7)。

1. Ananieva, O. et al. (2008) Nat Immunol 9, 1028-36.
2. Sury, M.D. et al. (2006) Free Radic Biol Med 41, 1372-83.
3. Duncan, E.A. et al. (2006) J Biol Chem 281, 12521-5.
4. Darragh, J. et al. (2005) Biochem J 390, 749-59.
5. Doehn, U. et al. (2004) Biochem J 382, 425-31.
6. Davie, J.R. (2003) Sci STKE 2003, PE33.
7. Soloaga, A. et al. (2003) EMBO J 22, 2788-97.
8. Wiggin, G.R. et al. (2002) Mol Cell Biol 22, 2871-81.
9. Caivano, M. and Cohen, P. (2000) J Immunol 164, 3018-25.
10. Tomás-Zuber, M. et al. (2001) J Biol Chem 276, 5892-9.
11. Tomás-Zuber, M. et al. (2000) J Biol Chem 275, 23549-58.

原厂资料：

Specificity / Sensitivity

MSK2 (D41A4) XP® Rabbit mAb detects endogenous levels of total MSK2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the region surrounding Pro751 of human MSK2.

Background

Mitogen- and stress-activated protein kinase 1 (MSK1) and MSK2 are serine/threonine kinases that promote immediate early gene transcription in stress- or mitogen-induced cells (1-4,7, 8) and LPS-stimulated macrophages (9). MSK2, also known as RSKB, contains two catalytic domains and has been shown to interact directly with p38 MAP kinase (10). MSK2 is phosphorylated and activated in response to tumor necrosis factor, epidermal growth factor or phorbol ester in HeLa cells or murine embryonic fibroblasts (MEFs) in a p38- and ERK-dependent manner (8,11). Phosphorylation on residues Ser196 and Thr568 within the activation loop of both catalytic domains is required for full kinase activation (11). Both MSK1 and MSK2 contain a functional nuclear localization sequence that is sufficient and required for nuclear targeting (10). Consistent with their nuclear localization, these kinases play an important role in regulating transcriptional responses to stress and mitogens. Activation of MSK2 in HeLa cells or MEFs results in rapid phosphorylation of histone H3, HMG-14, CREB and ATF1 and acetylation of histone H3 associated with immediate early gene transcription (3,4,6,7).

1. Ananieva, O. et al. (2008) Nat Immunol 9, 1028-36.
2. Sury, M.D. et al. (2006) Free Radic Biol Med 41, 1372-83.
3. Duncan, E.A. et al. (2006) J Biol Chem 281, 12521-5.
4. Darragh, J. et al. (2005) Biochem J 390, 749-59.
5. Doehn, U. et al. (2004) Biochem J 382, 425-31.
6. Davie, J.R. (2003) Sci STKE 2003, PE33.
7. Soloaga, A. et al. (2003) EMBO J 22, 2788-97.
8. Wiggin, G.R. et al. (2002) Mol Cell Biol 22, 2871-81.
9. Caivano, M. and Cohen, P. (2000) J Immunol 164, 3018-25.
10. Tomás-Zuber, M. et al. (2001) J Biol Chem 276, 5892-9.
11. Tomás-Zuber, M. et al. (2000) J Biol Chem 275, 23549-58.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.