p115 RhoGEF (D25D2) XP? Rabbit mAb

• 产品编号：CST-3669S      品牌：CST       原厂货号：3669S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

p115 RhoGEF (D25D2) XP® Rabbit mAb检测内源性p115 RhoGEF总蛋白。通过人工合成人源p115 RhoGEF蛋白氨基末端周围相应的多肽片段去免疫动物从而制备单克隆抗体。Rho family small GTPases包括Rho、Rac和cdc42，该家族作为分子开关，调节细胞进程例如细胞迁移、粘附、扩增和分化。它们通过鸟苷酸交换因子（guanine nucleotide exchange factor，GEF)被激活，这将催化已结合的GDP交换成GTP，然而通过GTPase activating proteins (GAPs)被抑制，这将催化GTP的水解成GDP。第三个调节水平通过Rho GDP dissociation inhibitor (RhoGDI)的计量结合力被提供(1)。在细胞表面G-protein coupled receptors (GPCRs)通过异质G 蛋白到small GTPases例如Rho从而发出信号，然而信号传递到下游效应分子 (2)。p115 RhoGEF/ArhGEF1和它的家族成员PDZ-RhoGEF (PRG)以及LARG通过异质G 蛋白被激活，因此从GPCRs到Rho small GTPases 的信号是成对(3-6)。在小鼠哮喘模型中，p115 RhoGEF蛋白对于T细胞进入气道炎症和其反应性是必须的 (7)。

1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
2. Morris, A.J. and Malbon, C.C. (1999) Physiol Rev 79, 1373-430.
3. Chen, Z. et al. (2003) J Biol Chem 278, 9912-9.
4. Hart, M.J. et al. (1998) Science 280, 2112-4.
5. Kozasa, T. et al. (1998) Science 280, 2109-11.
6. Bhattacharyya, R. and Wedegaertner, P.B. (2003) Biochem J 371, 709-20.
7. Brown, J.P. et al. (2007) Am J Respir Crit Care Med 176, 10-9.

原厂资料：

Specificity / Sensitivity

p115 RhoGEF (D25D2) XP® Rabbit mAb detects endogenous levels of total p115 RhoGEF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human p115 RhoGEF.

Background

The Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). 
 G-protein coupled receptors (GPCRs) at the cell surface signal through heteromeric G proteins to small GTPases such as Rho, which then signal to downstream effector molecules (2). p115 RhoGEF/ArhGEF1 and its family members PDZ-RhoGEF (PRG), and LARG are stimulated by heteromeric G proteins and thus couple signaling from GPCRs to Rho small GTPases (3-6). In a mouse model of asthma, p115 RhoGEF is necessary for T cells to enable airway inflammation and hyperreactivity (7).

1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
2. Morris, A.J. and Malbon, C.C. (1999) Physiol Rev 79, 1373-430.
3. Chen, Z. et al. (2003) J Biol Chem 278, 9912-9.
4. Hart, M.J. et al. (1998) Science 280, 2112-4.
5. Kozasa, T. et al. (1998) Science 280, 2109-11.
6. Bhattacharyya, R. and Wedegaertner, P.B. (2003) Biochem J 371, 709-20.
7. Brown, J.P. et al. (2007) Am J Respir Crit Care Med 176, 10-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.