Annexin A7 Antibody

• 产品编号：CST-3666S      品牌：CST       原厂货号：3666S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Annexin A7抗体识别内源性的全部Annexin A7蛋白，包括其亚型1和2。该多克隆抗体用与人类annexin A7蛋白羧基末端附近的氨基酸链对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和肽亲和层析纯化而得。Annexin（膜联蛋白）A7/ANXA7是钙/磷脂结合膜联蛋白家族的成员，参与膜融合过程和胞吐作用（1）。Annexin A7是一个GTP酶，与GTP的结合和PKC的活性都对调节蛋白功能非常重要（2,3）。Annexin A7与膜的结合是钙依赖的（4）。由于可变剪接的存在，它有两种异构体。Annexin A7的亚细胞定位已被证明是细胞质、囊泡结构、膜和肾上腺嗜铬颗粒（5,6）。其核定位被证明存在于发育小鼠中枢神经系统和成年小鼠的大脑中（7）。Annexin A7缺陷小鼠的研究表明，该蛋白在胰岛素分泌和钙信号通路中有作用（8），也与心肌细胞电活动稳定性中的细胞内钙稳态有关（9）。Annexin A7的基因是一个假定的肿瘤抑制基因（10），有报道发现其拷贝数改变在前列腺癌中发现（11）。Annexin A7的表达也与人类神经母细胞瘤患者的生存期有关（12），其单倍型不足在小鼠体内导致遗传不稳定，从而导致肿瘤的发生（13）。

1. Pollard, H.B. et al. (1990) J Membr Biol 117, 101-12.
2. Caohuy, H. and Pollard, H.B. (2002) J Biol Chem 277, 25217-25.
3. Caohuy, H. et al. (1996) Proc Natl Acad Sci USA 93, 10797-802.
4. Chander, A. et al. (2003) Cell Calcium 33, 11-7.
5. Selbert, S. et al. (1995) J Cell Sci 108 ( Pt 1), 85-95.
6. Clemen, C.S. et al. (2001) Neuroreport 12, 1139-44.
7. Rick, M. et al. (2005) BMC Neurosci 6, 25.
8. Srivastava, M. et al. (1999) Proc Natl Acad Sci USA 96, 13783-8.
9. Schrickel, J.W. et al. (2007) Cardiovasc Res 76, 257-68.
10. Srivastava, M. et al. (2001) Proc Natl Acad Sci USA 98, 4575-80.
11. Dong, J.T. (2006) J Cell Biochem 97, 433-47.
12. Hung, K.S. and Howng, S.L. (2003) J Neurosurg 99, 886-92.
13. Srivastava, M. et al. (2003) Proc Natl Acad Sci USA 100, 14287-92.

原厂资料：

Specificity / Sensitivity

Annexin A7 Antibody detects endogenous levels of total annexin A7 protein, including isoforms 1 and 2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human annexin A7. Antibodies are purified using protein A and peptide affinity chromatography.

Background

Annexin A7/ANXA7 is a member of the annexin family of calcium/phospholipid-binding proteins, and is involved in the process of membrane fusion and exocytosis (1). Annexin A7 is a GTPase, and both GTP-binding and PKC activity are important in regulating protein function (2,3). Membrane binding of annexin A7 is calcium dependent (4). Two isoforms exist due to alternative splicing. Subcellular localization of annexin A7 has been shown to be in the cytoplasm, vesicular structures, membrane and in adrenal chromaffin granules (5,6). Nuclear localization has been shown in the developing mouse central nervous system as well as in adult mouse brain (7). Annexin A7-deficient mouse studies show that the protein has a role in insulin secretion and calcium signaling (8) as well as cardiac intracellular calcium homeostasis electrical stability (9). The gene for annexin A7 is a putative tumor suppressor (10), and alterations in the copy number have been reported in prostate cancer (11). Annexin A7 expression has also been correlated with survival in human glioblastoma patients (12), and haploinsufficiency in mice may promote genetic instability leading to tumorigenesis (13).

1. Pollard, H.B. et al. (1990) J Membr Biol 117, 101-12.
2. Caohuy, H. and Pollard, H.B. (2002) J Biol Chem 277, 25217-25.
3. Caohuy, H. et al. (1996) Proc Natl Acad Sci USA 93, 10797-802.
4. Chander, A. et al. (2003) Cell Calcium 33, 11-7.
5. Selbert, S. et al. (1995) J Cell Sci 108 ( Pt 1), 85-95.
6. Clemen, C.S. et al. (2001) Neuroreport 12, 1139-44.
7. Rick, M. et al. (2005) BMC Neurosci 6, 25.
8. Srivastava, M. et al. (1999) Proc Natl Acad Sci USA 96, 13783-8.
9. Schrickel, J.W. et al. (2007) Cardiovasc Res 76, 257-68.
10. Srivastava, M. et al. (2001) Proc Natl Acad Sci USA 98, 4575-80.
11. Dong, J.T. (2006) J Cell Biochem 97, 433-47.
12. Hung, K.S. and Howng, S.L. (2003) J Neurosurg 99, 886-92.
13. Srivastava, M. et al. (2003) Proc Natl Acad Sci USA 100, 14287-92.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.