Integrin β5 (D24A5) Rabbit mAb

• 产品编号：CST-3629S      品牌：CST       原厂货号：3629S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Integrin beta-5(D24A5)兔源单抗检测内源性总的integrin beta-5蛋白水平。单抗由合成的肽段免疫动物产生，该肽段与人integrin beta-5蛋白的789位赖氨酸附近残基对应。Integrins是α/β异源二聚细胞表面受体，在细胞粘附和迁移，还有增长和存活中起关键作用（1,2）.整合素家族含至少18个α和8个β亚单元，可形成24个已知的整合素，它们带有独特组织分布特性和重叠的配基特异性（3）。整合素不仅在受到胞外环境刺激下，传递信号到细胞（由外而内信号），而且感知胞内因素来改变它们与胞外环境的相互作用(内到外的信号)（1,2）。αVβ5 integrin在各种组织和细胞类型中表达，包括内皮、上皮和成纤维细胞（4）。它在基质粘附到VN、FN、SPARC和骨唾液蛋白中起主要作用（5），并在神经胶质侵袭和转移癌细胞中起作用（6,7）。αVβ5 integrin在生长因子诱导的肿瘤血管生成中起主要作用，在那里被αVβ5 integrin协同信号传递，生长因子调控内皮细胞增殖和存活（8）。

1. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
2. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
4. Pasqualini, R. et al. (1993) J. Cell Sci. 105, 101-111.
5. Plow, E.F. et al. (2000) J. Biol. Chem. 275, 21785-21788.
6. Sun, L.C. et al. (2007) Cancer Lett. 246, 157-166.
7. Enns, A. et al. (2005) Eur. J. Cancer 41, 1065-1072.
8. Alghisi, G.C. and Rüegg, C. (2006) Endothelium 13, 113-135.

原厂资料：

Specificity / Sensitivity

Integrin beta-5 (D24A5) Rabbit mAb detects endogenous levels of total integrin beta-5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys789 of human integrin beta-5 protein.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins having distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2). 
 The αVβ5 integrin is expressed in various tissues and cell types, including endothelia, epithelia and fibroblasts (4). It plays a role in matrix adhesion to VN, FN, SPARC and bone sialoprotein (5) and functions in the invasion of gliomas and metastatic carcinoma cells (6,7). αVβ5 integrin plays a major role in growth-factor-induced tumor angiogenesis, where cooperative signaling by the αVβ5 integrin and growth factors regulates endothelial cell proliferation and survival (8).

1. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
2. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
4. Pasqualini, R. et al. (1993) J. Cell Sci. 105, 101-111.
5. Plow, E.F. et al. (2000) J. Biol. Chem. 275, 21785-21788.
6. Sun, L.C. et al. (2007) Cancer Lett. 246, 157-166.
7. Enns, A. et al. (2005) Eur. J. Cancer 41, 1065-1072.
8. Alghisi, G.C. and Rüegg, C. (2006) Endothelium 13, 113-135.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.