CDC37 (V297) Antibody

• 产品编号：CST-3618S      品牌：CST       原厂货号：3618S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CDC37 (V297) Antibody 能够检测内源性CDC37总蛋白。该多克隆抗体是由合成的人源的针对CDC37蛋白V297肽段免疫动物，利用A蛋白和多肽亲和层析方法纯化生产的。CDC37是HSP90伴侣复合体的重要组成部分(1-3)。CDC37蛋白含有一个氨基末端激酶结合结构域紧随中央的HSP90结合结构域。它能够阻止新合成的激酶肽链被降解并促进蛋白成熟的下一个步骤从而招募和稳定HSP90复合体中的激酶(4,5)。CDC37也抑制HSP90的ATP酶活性，从而导致复合体的构象变化阻止靶激酶的进入(6)。由于CDC37在多激酶通路中的重要作用如增殖和肿瘤细胞存活包括Raf, Akt, Src和ErbB2通路(7,8)，它一直被认为是重要的治疗靶点。

1. Karnitz, L.M. and Felts, S.J. (2007) Sci STKE 2007, pe22.
2. Caplan, A.J. et al. (2007) Trends Cell Biol 17, 87-92.
3. Caplan, A.J. et al. (2007) Cell Cycle 6, 3145-7.
4. Mandal, A.K. et al. (2007) J Cell Biol 176, 319-28.
5. Lee, P. et al. (2002) J Cell Biol 159, 1051-9.
6. Siligardi, G. et al. (2002) J Biol Chem 277, 20151-9.
7. Kimura, Y. et al. (1997) Genes Dev 11, 1775-85.
8. Gray, P.J. et al. (2008) Nat Rev Cancer 8, 491-5.

原厂资料：

Specificity / Sensitivity

CDC37 (V297) Antibody detects endogenous levels of total CDC37 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding V297 of human CDC37. Antibodies are purified by peptide affinity chromatography.

Background

CDC37 is an important component of the HSP90 chaperone complex (1,2). It was initially identified for its involvement in cell-cycle progression and was later found to have a much broader role as a chaperone for a wide variety of kinases and other proteins (1-3). CDC37 protein has an amino-terminal kinase binding domain followed by a central HSP90 binding domain. It recruits and stabilizes kinases in the HSP90 complex by protecting the newly synthesized kinase peptide chain from degradation and promoting the next step of protein maturation (4,5). CDC37 also suppresses the ATPase activity of HSP90, thereby leading to conformational changes in the complex that preclude target kinase loading (6). CDC37 has been proposed as a therapeutic target because of its important role in multiple kinase pathways involved in proliferation and cancer cell survival, including Raf, Akt, Src, and ErbB2 pathways (7,8).

1. Karnitz, L.M. and Felts, S.J. (2007) Sci STKE 2007, pe22.
2. Caplan, A.J. et al. (2007) Trends Cell Biol 17, 87-92.
3. Caplan, A.J. et al. (2007) Cell Cycle 6, 3145-7.
4. Mandal, A.K. et al. (2007) J Cell Biol 176, 319-28.
5. Lee, P. et al. (2002) J Cell Biol 159, 1051-9.
6. Siligardi, G. et al. (2002) J Biol Chem 277, 20151-9.
7. Kimura, Y. et al. (1997) Genes Dev 11, 1775-85.
8. Gray, P.J. et al. (2008) Nat Rev Cancer 8, 491-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.