Grp75 (D13H4) XP? Rabbit mAb

• 产品编号：CST-3593S      品牌：CST       原厂货号：3593S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Grp75 (D13H4) XP® Rabbit mAb兔单抗识别内源性的Grp75总蛋白。该单克隆抗体用与人类Grp75蛋白氨基酸序列对应的人工合成肽段免疫动物而制成。Grp75，也被称为mortalin，是Hsp70分子伴侣蛋白家族的成员，但不是热诱导的（1,2）。此蛋白对许多线粒体蛋白从细胞质到线粒体的转运至关重要（3）。Grp75可以使肿瘤抑制因子p53失活（4）。研究发现Grp75在很多肿瘤组织中过表达，使人类细胞系永生化，提示它在肿瘤形成中具有作用（5）。Grp75也与细胞衰老有关，因为其过表达似乎能够延长人类成纤维细胞的生存期（6）。

1. Kaul, S.C. et al. (1993) Biochem Biophys Res Commun 193, 348-55.
2. Wadhwa, R. et al. (1993) J Biol Chem 268, 6615-21.
3. Schneider, H.C. et al. (1994) Nature 371, 768-74.
4. Wadhwa, R. et al. (1998) J Biol Chem 273, 29586-91.
5. Wadhwa, R. et al. (2006) Int J Cancer 118, 2973-80.
6. Kaul, S.C. et al. (2003) Exp Cell Res 286, 96-101.

原厂资料：

Specificity / Sensitivity

Grp75 (D13H4) XP® Rabbit mAb detects endogenous levels of total Grp75 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Grp75.

Background

Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6).

1. Kaul, S.C. et al. (1993) Biochem Biophys Res Commun 193, 348-55.
2. Wadhwa, R. et al. (1993) J Biol Chem 268, 6615-21.
3. Schneider, H.C. et al. (1994) Nature 371, 768-74.
4. Wadhwa, R. et al. (1998) J Biol Chem 273, 29586-91.
5. Wadhwa, R. et al. (2006) Int J Cancer 118, 2973-80.
6. Kaul, S.C. et al. (2003) Exp Cell Res 286, 96-101.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.