CD4 (Edu-2) Mouse mAb

• 产品编号：CST-3563S      品牌：CST       原厂货号：3563S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CD4 (Edu-2) Mouse mAb 鼠单抗能够检测内源CD4蛋白总体水平。此单克隆抗体是通过刺激人的粒性白细胞来免疫BALB/c 小鼠而获得。CD44 是I类跨膜糖蛋白，能够介导细胞-细胞和细胞-基质通过对透明质酸(HA)的亲和性或细胞外基质(ECM)而相互作用。CD44 具有多种形态，多种剪切形式，并且具有多样的转录后修饰(1,2)。细胞表面CD44表达的增加是T细胞激活的特征之一，并且此蛋白在炎症应答过程中上调表达。CD44与HER2 的相互作用与卵巢癌细胞的生长有关(1-3)。CD44 与埃兹蛋白、根蛋白和膜突蛋白(ERM)的相互作用与细胞骨架连接到细胞质膜和ECM有关(4-6)。在静息细胞中，CD44是组成型在 Ser325位点磷酸化。 PKC的激活使Ser291位点磷酸化,Ser325位点去磷酸化, 导致埃兹蛋白和CD44蛋白分离和定向运动(4)。

Zamoyska, R. (1994) Immunity 1, 243-246.

原厂资料：

Specificity / Sensitivity

CD4 (Edu-2) Mouse mAb detects endogenous levels of total CD4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes.

Background

Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

Zamoyska, R. (1994) Immunity 1, 243-246.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.