Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb

• 产品编号：CST-3556S      品牌：CST       原厂货号：3556S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb兔单抗能检测内源性Ser227位点磷酸化的RSK2蛋白水平。该抗体也能与同源丝氨酸残基磷酸化的RSK1蛋白发生交叉反应。该单克隆抗体通过使用与人源RSK2蛋白Ser227位点周围残基相一致的合成磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。90kDa核糖体S6激酶(RSK1-4)是一个广泛表达的丝/苏氨酸激酶家族，其特征是包含2个不同的功能性激酶结构域（1）和一个羧基端的细胞外信号调节激酶（ERKs）结合位点（2）。RSK激酶结构域内或以外的一些位点，包括Ser380, Thr359, Ser363和Thr573，对于激酶的激活非常重要（3）。RSK1-3在多种生长因子、多肽激素和神经递质的刺激下，通过MAPKs的磷酸化作用、自磷酸化作用以及PI3K的信号途径的协同作用被激活（3）。在体内及体外，PDK1都能磷酸化RSK2胺端激酶区域内活化环的Ser227位点，导致激酶的大量活化。在经过生长因子或UV处理的细胞中，组成型活化的PDK1与ERK协同作用，激活RSK(4, 5)。

1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
4. Jensen, C.J. et al. (1999) J Biol Chem 274, 27168-76.
5. Mérienne, K. et al. (2000) Oncogene 19, 4221-9.

原厂资料：

Specificity / Sensitivity

Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb detects endogenous levels of RSK2 only when phosphorylated at Ser227. It shows cross-reactivity with RSK1 when phosphorylated at the homologous serine residues.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser227 of human RSK2.

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
4. Jensen, C.J. et al. (1999) J Biol Chem 274, 27168-76.
5. Mérienne, K. et al. (2000) Oncogene 19, 4221-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.