CD31 (PECAM-1) (89C2) Mouse mAb

• 产品编号：CST-3528S      品牌：CST       原厂货号：3528S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CD31 (PECAM-1) (89C2) Mouse mAb 鼠单抗能够检测内源水平CD31蛋白总体水平。此抗体不与其它的相关蛋白交叉反应。此单克隆抗体是通过合成人源CD31的融合蛋白来来免疫动物而获得。CD31 (血小板内皮细胞粘附分子-1: PECAM-1),是Ig粘附分子家族成员之一，它在循环血小板，单核细胞，中性粒细胞，有些T细胞和血管内皮细胞中表达，并调节细胞粘附，迁移和血管内皮细胞(1)。CD31在受到很有可能是Src家族成员的多种刺激 (如：机械应力或氧化应激)后，能够在细胞质C-末端尾巴的Tyr686位点磷酸化(2)。酪氨酸磷酸化介导的与很多含有SH2结合域的伴侣蛋白相关，如PI3激酶, SHIP, PLCγ 和 SHP-2。因此，CD31作为很多信号分子的脚手架(3)。

1. Newman, P.J. (1997) J. Clin. Invest. 99, 3-8.
2. Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-15772.
3. Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-524.

原厂资料：

Specificity / Sensitivity

CD31 (PECAM-1) (89C2) Mouse mAb detects endogenous levels of total CD31 protein. It does not cross-react with other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human CD31 protein.

Background

CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells and endothelial cells, and modulates cell adhesion, endothelial cell migration and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical stress or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain containing binding partners such as PI3-kinase, SHIP, PLCγ and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).

1. Newman, P.J. (1997) J. Clin. Invest. 99, 3-8.
2. Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-15772.
3. Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-524.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.