Phospho-MDM2 (Ser166) Antibody

• 产品编号：CST-3521S      品牌：CST       原厂货号：3521S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MDM2 (Ser166) Antibody能够检测内源性丝氨酸（166位）磷酸化的MDM2蛋白。该多克隆抗体是由合成的人源的针对MDM2蛋白丝氨酸（166位）的磷酸化肽段免疫动物，采用蛋白A和多肽亲和层析技术生产的。MDM2，一种p53泛素连接酶，在p53稳定性的调节中至关重要(1)。Akt介导的MDM2苏氨酸166和168位磷酸化增加了它和p300的相互作用，允许MDM2介导的泛素化和p53降解(2-4)。MDM2的磷酸化也阻碍其结合p19ARF，促进p53降解(3)。

1. Haupt, Y. et al. (1997) Nature 387, 296-299.
2. Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
3. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
4. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.

原厂资料：

Specificity / Sensitivity

Phospho-MDM2 (Ser166) Antibody detects endogenous levels of MDM2 only when phosphorylated at Ser166.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser166 of human MDM2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).

1. Haupt, Y. et al. (1997) Nature 387, 296-299.
2. Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
3. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
4. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.