Phospho-Keratin 17 (Ser44) Antibody

• 产品编号：CST-3519S      品牌：CST       原厂货号：3519S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Keratin 17 (Ser44) 抗体仅识别Ser44磷酸化的内源性keratin 17总蛋白。通过人工合成与人源keratin 17蛋白Ser44邻近氨基酸序列一致的肽段，免疫动物制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。角蛋白（细胞角蛋白）是中间丝蛋白主要在上皮细胞中表达。角蛋白的异二聚体由一个酸性角蛋白（或type I角蛋白，角蛋白9-23或）和一个碱性角蛋白（type II角蛋白，角蛋白1-8）聚合形成中间丝（1.2）。角蛋白的亚型在组织-和分化的特异性表达，这使他们可做为有用的生物标志（1）。研究显示角蛋白基因突变与肌肤失调，肝脏和胰腺疾病，以及肠道炎性疾病相关（3-6）。Keratin 17蛋白已经显示涉及伤口愈合，这是一个需要快速的细胞骨架重组的过程(7)。另外需要细胞骨架重组的过程是细胞生长。研究证明在keratin 17敲除的角质细胞中通过Akt/mTOR通路的信号不能产生在翻译、细胞大小或生长中的增加，并且这个缺失与14-3-3σ蛋白的不正常定位有关联。自从在正常细胞中14-3-3σ蛋白与keratin 17蛋白有关联，一个模式被提出：在细胞质中通过Akt/mTOR通路的信号产生一个 14-3-3σ蛋白的隔离，这凭借它与keratin 17蛋白相互作用，并且通过keratin 17蛋白的这个隔离对翻译和细胞生长是需要的。keratin 17蛋白在Ser 44位点磷酸化被认为提供了一个14-3-3σ结合的停留位点(8)。

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
7. Paladini, R.D. et al. (1996) J Cell Biol 132, 381-97.
8. Kim, S. et al. (2006) Nature 441, 362-5.

原厂资料：

Specificity / Sensitivity

Phospho-Keratin 17 (Ser44) Antibody detects endogenous levels of keratin 17 only when phosphorylated on Ser44.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids surrounding Ser44 of human keratin 17. Antibodies are purified by Protein A and peptide affinity chromatography.

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

Keratin 17 has been shown to be involved in wound healing, a process that requires rapid remodelling of the cytoskeleton (7). Another process that requires cytoskeletal remodelling is cell growth. It has been shown that in keratin 17 null keratinocytes that signaling through the Akt/mTOR pathway fails to produce an increase in translation, cell size or growth, and that this defect is associated with abnormal localization of 14-3-3σ. Since in normal cells, 14-3-3σ associates with keratin 17, a model has been proposed whereby signaling through Akt/mTOR produces a sequestration of 14-3-3σ in the cytosol via its interaction with keratin 17, and this sequestration by keratin 17 is required for translation and cell growth. Phosphorylation of keratin 17 on Ser 44 is thought to provide a docking site for 14-3-3σ binding (8).

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
7. Paladini, R.D. et al. (1996) J Cell Biol 132, 381-97.
8. Kim, S. et al. (2006) Nature 441, 362-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.