MLH1 (4C9C7) Mouse mAb

• 产品编号：CST-3515S      品牌：CST       原厂货号：3515S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MLH1 (4C9C7) Mouse mAb能够检测内源性MLH1总蛋白。该单克隆抗体是由重组的MBP-MLH1截短体免疫动物生产的。错配修复(MMR)，一种涉及DNA合成过程中错误纠正的保守过程，对于维持基因组的完整性至关重要。MLH1是大肠杆菌MMR基因突变体的人类同源蛋白。MMR识别碱基错配或者插入/缺失回路需要MutL异源二聚体复合物招募而来的MutS同系物，该复合体由MLH1 和PMS1 (MutL-α), PMS2 (MutL-β)或者MLH3 (MutL-γ)组成。其它影响真核细胞中MMR的因子有EXO1, PCNA, RFC, RPA, DNA 聚合酶和DNA连接酶(回顾1)。MLH1基因失活可以引起基因组不稳定性和肿瘤易感性(2-5)。遗传性非息肉型结肠癌(HNPCC)中MLH1基因突变频繁(6)。MLH1也在减数分裂重组中起重要作用(7)。

1. Modrich, P. (2006) J Biol Chem 281, 30305-9.
2. Seng, T.J. et al. (2008) Br J Cancer 99, 375-82.
3. Harley, I. et al. (2008) Gynecol Oncol 109, 384-7.
4. Mao, G. et al. (2008) J Biol Chem 283, 3211-6.
5. Hubner, R.A. and Houlston, R.S. (2007) J Natl Cancer Inst 99, 1490; author reply 1490-1.
6. Vasen, H.F. (2005) Fam Cancer 4, 219-25.
7. Argueso, J.L. et al. (2003) Mol Cell Biol 23, 873-86.

原厂资料：

Specificity / Sensitivity

MLH1 (4C9C7) Mouse mAb detects endogenous levels of total MLH1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with truncated recombinant MBP-MLH1.

Background

Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-α), PMS2 (MutL-β) or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7).

1. Modrich, P. (2006) J Biol Chem 281, 30305-9.
2. Seng, T.J. et al. (2008) Br J Cancer 99, 375-82.
3. Harley, I. et al. (2008) Gynecol Oncol 109, 384-7.
4. Mao, G. et al. (2008) J Biol Chem 283, 3211-6.
5. Hubner, R.A. and Houlston, R.S. (2007) J Natl Cancer Inst 99, 1490; author reply 1490-1.
6. Vasen, H.F. (2005) Fam Cancer 4, 219-25.
7. Argueso, J.L. et al. (2003) Mol Cell Biol 23, 873-86.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.