Sav1 Antibody

• 产品编号：CST-3507S      品牌：CST       原厂货号：3507S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Sav1抗体识别内源性的总Sav1蛋白。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源Sav1蛋白Ala100的氨基酸残基虚列一致，抗体由蛋白A和肽段亲和层析技术纯化。Sav1（Salvador同源物1）最早被定义为含有一对WW结构域和一个卷曲螺旋区域的45kDa蛋白。起初命名为WW45，Sav1在成人组织广泛表达，但也在特异发育时期存在，Sav1在包括MST2和LATS1激酶，以及YAP转录共激活复合物中扮演骨架蛋白（3）。这组蛋白在最近确定的Hippo通路中起功能，Hippo通路调节重要发育功能比如细胞接触抑制和器官大小。进一步证据显示在肿瘤抑制和癌症病理方面起作用（4,5）。Hippo通路需要Sav1 对MST1的激活和导致LATS1/2的激活（6）。胚胎小鼠缺失Sav1显示肥大增生和表皮分化缺失，暗示Sav1在表皮细胞分化有重要作用（7）。

1. Valverde, P. (2000) Biochem Biophys Res Commun 276, 990-8.
2. Oka, T. et al. (2008) J Biol Chem 283, 27534-46.
3. Guo, C. et al. (2007) Curr Biol 17, 700-5.
4. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
5. Tapon, N. et al. (2002) Cell 110, 467-78.
6. Lee, J.H. et al. (2008) EMBO J 27, 1231-42.
7. Donninger, H. et al. (2011) J Biol Chem 286, 18483-91.

原厂资料：

Specificity / Sensitivity

Sav1 Antibody detects endogenous level of total Sav1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala100 of human Sav1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Sav1 (Salvador homolog 1) was first identified as a 45 kDa protein containing a pair of WW domains and a coiled-coil region. Originally named WW45, Sav1 is ubiquitously expressed in adult tissues but is also present during specific developmental periods. Sav1 acts a scaffold protein in a complex that includes the kinases MST2 and LATS1, and a YAP transcriptional co-activator (3). This group of proteins functions in the newly identified Hippo pathway, which regulates important developmental functions such as cell contact inhibition and organ size. Further evidence suggests a role in tumor suppression and cancer pathogenesis (4,5). The Hippo pathway requires Sav1 for activation of MST1 and the resulting activation of LATS1/2 (6). Embryonic mice lacking Sav1 display hyperplastic growth and epithelial differentiation defects, implying that Sav1 plays a prominent role epithelial cell differentiation (7).

1. Valverde, P. (2000) Biochem Biophys Res Commun 276, 990-8.
2. Oka, T. et al. (2008) J Biol Chem 283, 27534-46.
3. Guo, C. et al. (2007) Curr Biol 17, 700-5.
4. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
5. Tapon, N. et al. (2002) Cell 110, 467-78.
6. Lee, J.H. et al. (2008) EMBO J 27, 1231-42.
7. Donninger, H. et al. (2011) J Biol Chem 286, 18483-91.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.