JMJD3 Antibody

• 产品编号：CST-3457S      品牌：CST       原厂货号：3457S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

JMJD3 Antibody能够检测内源性JMJD蛋白水平。通过合成的与人源JMJD3蛋白相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。组蛋白赖氨酸的甲基化水平对于基因组的活化和非活化区域的形成是主要决定因素，并且在发育期间对于基因组的正确进程起着关键作用(1,2)。包含蛋白质的Jumonji C (JmjC)区域代表最大的潜在组蛋白去乙酰化酶蛋白(3)。JmjC区域通过氧化反应能催化单、双和三甲基化的赖氨酸残基的去乙甲基化，这种氧化反应需要铁离子和α-酮戊二酸(3)。基于同源性，人源和小鼠都包含至少30种这样蛋白质，这能够被分为7个不同的家族(3)。UTX/UTY家族的三个成员包含ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)、ubiquitously transcribed Y chromosome tetratricopeptide repeat protein (UTY)和JmjC domain-containing protein 3 (JMJD3) (3)。这个蛋白家族已经显示使双核三甲基化的histone H3蛋白Lys 27位点去甲基化(4-8)。在女性中UTX基因逃避X 染色体失活(X inactivation)，并且广泛表达(9)。在发育期间UTX的功能是去调节HOX基因表达(4-6)。JMJD3的功能是去调节在巨噬细胞中不同炎症刺激下的基因表达，并且显示在前列腺癌中上调(7,8)。UTX和JMJD3蛋白与mixed-lineage leukemia (MLL)复合物2和3相互作用，两者都已经显示使histone H3蛋白Lys4 位点甲基化(6,7)。UTY基因在雄性小鼠的许多组织中表达(10)。在很大程度上，UTY蛋白的功能还不清楚。

1. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
2. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
3. Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27.
4. Agger, K. et al. (2007) Nature 449, 731-4.
5. Lan, F. et al. (2007) Nature 449, 689-94.
6. Lee, M.G. et al. (2007) Science 318, 447-50.
7. De Santa, F. et al. (2007) Cell 130, 1083-94.
8. Xiang, Y. et al. (2007) Cell Res 17, 850-7.
9. Greenfield, A. et al. (1998) Hum Mol Genet 7, 737-42.
10. Greenfield, A. et al. (1996) Nat Genet 14, 474-8.

原厂资料：

Specificity / Sensitivity

JMJD3 Antibody detects transfected levels of JMJD3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human JMJD3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). The three members of the UTX/UTY family include the ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX), the ubiquitously transcribed Y chromosome tetratricopeptide repeat protein (UTY) and JmjC domain-containing protein 3 (JMJD3) (3). This family of proteins has been shown to demethylate both di- and tri-methyl histone H3 Lys 27 (4-8). The UTX gene escapes X inactivation in females and is ubiquitously expressed (9). UTX functions to regulate HOX gene expression during development (4-6). JMJD3 functions to regulate gene expression in macrophages responding to various inflammatory stimuli and has been shown to be upregulated in prostate cancer (7,8). Both UTX and JMJD3 interact with mixed-lineage leukemia (MLL) complexes 2 and 3, both of which have been shown to methylate histone H3 at Lys4 (6,7). The UTY gene is expressed in most tissues in the male mouse (10). The function of UTY is largely unknown.

1. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
2. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
3. Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27.
4. Agger, K. et al. (2007) Nature 449, 731-4.
5. Lan, F. et al. (2007) Nature 449, 689-94.
6. Lee, M.G. et al. (2007) Science 318, 447-50.
7. De Santa, F. et al. (2007) Cell 130, 1083-94.
8. Xiang, Y. et al. (2007) Cell Res 17, 850-7.
9. Greenfield, A. et al. (1998) Hum Mol Genet 7, 737-42.
10. Greenfield, A. et al. (1996) Nat Genet 14, 474-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.