Rabbit IgG Isotype Control (Alexa Fluor? 647 Conjugate)

产品编号：CST-3452S 品牌：CST 原厂货号：3452S
产品分类：抗体 > 抗体制备 > 同型抗体对照Isotype controls
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4°C

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描述：

纯化的抗体不能直接用于其它已知抗原的检测，其功能是作为兔源抗体的同型对照。它是从天然兔血浆中分离出来的，通过亲和色谱法制备，最佳条件下采用Alexa Fluor ® 647荧光染料标记，其F/P值为2-6。使用时注意：该同型对照抗体在分析时应该与被测抗体具有相同的浓度。如果兔源IgG同型对照过量会形成较强的背景。请不要再使用此抗体时与CST Alexa Fluor® conjugated antibodies一样采用建议的1:50的稀释度。

Rabbit IgG在最佳条件下采用Alexa Fluor ® 647荧光染料标记，已经过内部测试，可以直接用于人类细胞和小鼠细胞的流式细胞分析。 The Alexa Fluor ® 647 染料可以被红光激发（比如 633 nm He-Ne 激光），与Alexa Fluor ® 647 染料结合的抗体可以产生明亮的远红荧光，最大峰值在665nm。

同型对照是用来评估针对由于Fc受体结合或者其它蛋白与蛋白之间的相互作用引起的一抗的非特异性结合。一个同型对照抗体应该与被测抗体具有相同的免疫球蛋白类型和相同的浓度。

原厂资料：

Specificity / Sensitivity

The purified antibody is not directed against any known antigen. It functions as an isotype control for rabbit antibodies.

Source / Purification

The antibody was isolated from naive rabbit sera and purified by affinity chromotography. The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with a F/P ratio of 2-6.Important Note on Use: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. Higher background fluorescence may result if excessive amounts of rabbit IgG isotype control are used. Do not use this antibody at 1:50 dilution in the same way as recommended for other CST Alexa Fluor® conjugated antibodies.

Description

Rabbit IgG was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions, and tested in-house for direct flow cytometric analysis of human and mouse cells. Alexa Fluor® dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugated of the Alexa Fluor® 647 dye produce far-red-fluorescence emission with a peak at 655 nm.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.