Phospho-μ-Opioid Receptor (Ser375) Antibody

• 产品编号：CST-3451S      品牌：CST       原厂货号：3451S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-μ-Opioid Receptor (Ser375) Antibody 兔多抗识别转染水平的鼠MOR Ser375磷酸化的（或人MOR Ser377磷酸化的）μ-opioid受体。该多克隆抗体由合成的人类μ-opioid受体Ser377位点（与鼠Ser375同源）附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。μ-阿片受体（MOR）属于G蛋白偶联受体家族。MOR介导吗啡和其他阿片类药物以及几种内源性阿片肽的镇痛和成瘾效应（1）。与配体结合后，Gi偶联受体灭活腺苷酸环化酶（1），激活许多G-β-γ-依赖的途径，包括MAPK和PI3K/Akt信号级联（2,3）。这些受体在质膜和相关目的地之间的转运在吗啡耐受中扮演重要的角色（4,5）。与其他GPCRs一样，这些过程也被胞内域不同位点的磷酸化所调控（6）。在各种位点中，激动剂特异的磷酸化MOR小鼠丝氨酸375（人类丝氨酸377）是内化所必需的（7）。

1. Law, P. Y. et al. (2000) Annu. Rev. Pharmacol. Toxicol. 40, 389-430.
2. Polakiewicz, R. D. et al. (1998) J. Biol. Chem. 273, 12402-12406.
3. Polakiewicz, R. D. et al. (1998) J. Biol. Chem. 273, 23534-23541.
4. Finn, A.K. and Whistler, J.L. (2001) Neuron 32, 829-839.
5. Kieffer, B.L. and Evans, C.J. (2002) Cell 108, 587-590.
6. Yu, Y. et al. (1997) J. Biol. Chem. 272, 28869-28874.
7. El Kouhen, R. et al. (2001) J. Biol. Chem. 276, 12774-12780.

原厂资料：

Specificity / Sensitivity

Phospho-µ-Opioid Receptor (Ser375) Antibody detects transfected µ-opioid receptor only when phosphorylated at Ser375 of mouse MOR (or Ser377 of human MOR).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser377 of human (homologous to Ser375 of mouse) μ-opioid receptor. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The μ-opioid receptor (MOR) belongs to the superfamily of G-protein-coupled receptors. MOR mediates the analgesic and rewarding effects of morphine and other opiates as well as the actions of several endogenous opioid peptides (1). Upon binding to its ligands, this Gi-coupled receptor inactivates adenylyl cyclase (1) and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades (2,3). Trafficking of these receptors to and from the plasma membrane and their desensitization play a significant role in morphine tolerance (4,5). As with other GPCRs, these processes are modulated by phosphorylation at diverse sites within intracellular domains (6). Among other sites, agonist-specific phosphorylation of serine 375 in mouse (serine 377 in human) MOR is essential for its internalization (7).

1. Law, P. Y. et al. (2000) Annu. Rev. Pharmacol. Toxicol. 40, 389-430.
2. Polakiewicz, R. D. et al. (1998) J. Biol. Chem. 273, 12402-12406.
3. Polakiewicz, R. D. et al. (1998) J. Biol. Chem. 273, 23534-23541.
4. Finn, A.K. and Whistler, J.L. (2001) Neuron 32, 829-839.
5. Kieffer, B.L. and Evans, C.J. (2002) Cell 108, 587-590.
6. Yu, Y. et al. (1997) J. Biol. Chem. 272, 28869-28874.
7. El Kouhen, R. et al. (2001) J. Biol. Chem. 276, 12774-12780.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.