Flotillin-2 (C42A3) Rabbit mAb

• 产品编号：CST-3436S      品牌：CST       原厂货号：3436S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Flotillin-2 (C42A3) Rabbit mAb识别内源性的flotillin-2总蛋白。通过合成与人源flotillin-2蛋白Phe157邻近氨基酸序列一致的肽段，免疫动物从而制备出单克隆抗体。Flotillins蛋白属于一个脂筏相关内在膜蛋白家族，其拥有一个进化上保守区域又称为prohibitin homology domain (PHB)(1)。Flotillin蛋白成员是普遍表达，并且位于细胞质膜上非囊泡（脂筏），而这是它们传递信号和调节脂筏运动和定位场所(2-5)。flotillin-1和flotillin-2两个flotillin蛋白成员已经被描述。除了在细胞质膜上与脂筏共定位之外，flotillin-1蛋白也已经被发现在内吞作用和自噬体通路中，例如再循环内体/次级内体、高尔基体复合物和细胞核(6,7)。Flotillin-2蛋白主要位于细胞质膜以及普遍存在于细胞与细胞连接处。然而，flotillin-2蛋白的过表达已经发现在次级内体(4,8,9)。flotillin-1和flotillin-2蛋白都共同被用作脂筏相关的标记物。

1. Morrow, I.C. and Parton, R.G. (2005) Traffic 6, 725-40.
2. Langhorst, M.F. et al. (2005) Cell Mol Life Sci 62, 2228-40.
3. Stuermer, C.A. and Plattner, H. (2005) Biochem Soc Symp , 109-18.
4. Fernow, I. et al. (2007) Eur J Cell Biol 86, 345-52.
5. Neumann-Giesen, C. et al. (2007) J Cell Sci 120, 395-406.
6. Liu, J. et al. (2005) J Biol Chem 280, 16125-34.
7. Santamaría, A. et al. (2005) Mol Cell Biol 25, 1900-11.
8. Solomon, S. et al. (2002) Immunobiology 205, 108-19.
9. Neumann-Giesen, C. et al. (2004) Biochem J 378, 509-18.

原厂资料：

Specificity / Sensitivity

Flotillin-2 (C42A3) Rabbit mAb detects endogenous levels of total flotillin-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe157 of human flotillin-2 protein.

Background

Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry a evolutionarily conserved domain called the prohibitin homology domain (PHB) (1). Flotillin members are ubiquitously expressed and located to non-caveolar microdomains (lipid rafts) on the cell plasma membrane where they support signal transduction and regulate lipid raft motility and localization (2-5). Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found at compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, as well as the nucleus (6,7). Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are commonly used as lipid raft associated markers.

1. Morrow, I.C. and Parton, R.G. (2005) Traffic 6, 725-40.
2. Langhorst, M.F. et al. (2005) Cell Mol Life Sci 62, 2228-40.
3. Stuermer, C.A. and Plattner, H. (2005) Biochem Soc Symp , 109-18.
4. Fernow, I. et al. (2007) Eur J Cell Biol 86, 345-52.
5. Neumann-Giesen, C. et al. (2007) J Cell Sci 120, 395-406.
6. Liu, J. et al. (2005) J Biol Chem 280, 16125-34.
7. Santamaría, A. et al. (2005) Mol Cell Biol 25, 1900-11.
8. Solomon, S. et al. (2002) Immunobiology 205, 108-19.
9. Neumann-Giesen, C. et al. (2004) Biochem J 378, 509-18.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.