RECK (D8C7) Rabbit mAb

• 产品编号：CST-3433S      品牌：CST       原厂货号：3433S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RECK (D8C7)兔源单抗检测内源性总的RECK 蛋白。单抗由合成肽段免疫动物产生，该肽段与人RECK 蛋白913位异亮氨酸附近残基相对应。RECK（逆转诱导的带有Kazal基序半胱氨酸富集蛋白质）是一个GPI锚定的膜糖蛋白，它负向调控基质金属蛋白酶（MMP）家族成员，起着转化抑制因子的作用（1,2）。它在MMP抑制中的功能使得RECK在发育中成为调控胞外基质形成和稳定的一个关键因子（2-4）。RECK也与其它胞外基质蛋白酶比如ADAM10和CD13有关，并在调节靶标蛋白吞噬现象和Notch信号中起作用（5,6）。RECK广泛在正常组织中表达，因启动子甲基化引起的RECK表达的减少与肿瘤转化、血管生成和转移有关（10,11）。

1. Takahashi, C. et al. (1998) Proc Natl Acad Sci USA 95, 13221-6.
2. Oh, J. et al. (2001) Cell 107, 789-800.
3. Kondo, S. et al. (2007) J Cell Sci 120, 849-57.
4. Echizenya, M. et al. (2005) Oncogene 24, 5850-7.
5. Miki, T. et al. (2007) J Biol Chem 282, 12341-52.
6. Muraguchi, T. et al. (2007) Nat Neurosci 10, 838-45.
7. Noda, M. et al. Cancer Metastasis Rev 22, 167-75.
8. Long, N.K. et al. (2008) Oral Oncol 44, 1052-8.
9. Chang, H.C. et al. (2007) Cancer Sci 98, 169-73.
10. Clark, J.C. et al. (2007) Cancer Metastasis Rev 26, 675-83.
11. Noda, M. and Takahashi, C. (2007) Cancer Sci 98, 1659-65.

原厂资料：

Specificity / Sensitivity

RECK (D8C7) Rabbit mAb detects endogenous levels of total RECK protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile913 of human RECK protein.

Background

RECK (reversion-inducing cysteine-rich protein with Kazal motif) is a GPI-anchored membrane glycoprotein that negatively regulates members of the matrix metalloproteinase (MMP) family and functions as a suppressor of transformation (1,2). Its function in MMP inhibition makes RECK a crucial factor in the regulation of extracellular matrix formation and stability during development (2-4). RECK has also been linked to the regulation of other extracellar matrix proteases such as ADAM10 and CD13 and functions in modulating target protein endocytosis and Notch signaling (5,6). RECK is widely expressed in normal tissue and decreased expression of RECK due to promoter methylation has been correlated with tumor transformation, angiogenesis and metastasis (1,7-9). Therefore, loss of RECK expression serves as a prognostic hallmark for cancer malignancy (10,11).

1. Takahashi, C. et al. (1998) Proc Natl Acad Sci USA 95, 13221-6.
2. Oh, J. et al. (2001) Cell 107, 789-800.
3. Kondo, S. et al. (2007) J Cell Sci 120, 849-57.
4. Echizenya, M. et al. (2005) Oncogene 24, 5850-7.
5. Miki, T. et al. (2007) J Biol Chem 282, 12341-52.
6. Muraguchi, T. et al. (2007) Nat Neurosci 10, 838-45.
7. Noda, M. et al. Cancer Metastasis Rev 22, 167-75.
8. Long, N.K. et al. (2008) Oral Oncol 44, 1052-8.
9. Chang, H.C. et al. (2007) Cancer Sci 98, 169-73.
10. Clark, J.C. et al. (2007) Cancer Metastasis Rev 26, 675-83.
11. Noda, M. and Takahashi, C. (2007) Cancer Sci 98, 1659-65.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.