Cytokine Receptor Common beta-Chain Antibody

• 产品编号：CST-3432S      品牌：CST       原厂货号：3432S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cytokine Receptor Common beta-Chain 抗体能够检测内源cytokine receptor common beta-chain蛋白总体水平并且不依赖于磷酸化。多克隆抗体是通过合成人源对应的细胞因子受体common beta-chain C-末端周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。粒细胞巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-3 (IL-3) 和IL-5共有的beta-c链是这些受体的重要信号传递单元, 与配体结合从而具有生物活性(1-3)。beta-c链的酪氨酸磷酸化是GM-CSF, IL-3 和IL-5受体激活和信号抑制过程中的第一步(4)。beta-c链的14-3-3结合序列内的丝氨酸磷酸化也参与到了GM-CSF, IL-3 和IL-5 受体特异性的作用中(5,6)。

1. Kitamura, T. et al. (1991) Cell 66, 1165-74.
2. Tavernier, J. et al. (1991) Cell 66, 1175-1184.
3. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 9655-9659.
4. Muto, A. et al. (1996) J. Exp. Med. 183, 1911-1926.
5. Stomski, F.C. et al. (1999) Blood 94, 1933-42.
6. Guthridge, M.A. et al. (2000) Mol. Cell 6, 99-108.

原厂资料：

Specificity / Sensitivity

Cytokine Receptor Common beta-Chain Antibody detects endogenous levels of total cytokine receptor common beta-chain protein independent of phosphorylation.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human cytokine receptor common beta-chain. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The common beta-chain (beta-c) of the granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) and IL-5 receptors is the major signaling subunit of these receptors, coupling ligand binding to multiple biological activities (1-3). Tyrosine phosphorylation of cytokine receptor common beta-chain is one of the first events in GM-CSF, IL-3 and IL-5 receptor activation and in signaling initiation (4). Serine phosphorylation within the 14-3-3 binding sequence of the common beta-chain is also involved in GM-CSF, IL-3 and IL-5 receptor-specific functions (5,6).

1. Kitamura, T. et al. (1991) Cell 66, 1165-74.
2. Tavernier, J. et al. (1991) Cell 66, 1175-1184.
3. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 9655-9659.
4. Muto, A. et al. (1996) J. Exp. Med. 183, 1911-1926.
5. Stomski, F.C. et al. (1999) Blood 94, 1933-42.
6. Guthridge, M.A. et al. (2000) Mol. Cell 6, 99-108.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.