NHERF1 (A140) Antibody

• 产品编号：CST-3382S      品牌：CST       原厂货号：3382S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NHERF1 (A140) Antibody 兔多抗识别内源性的总NHERF1蛋白。该多克隆抗体由合成的人类NHERF1蛋白Ala140附近肽段免疫动物而制成。该抗体使用多肽亲和层析纯化而得。Na+/H+交换泵调节因子(NHERF1 or EBP-50)是多个包含PDZ结构域的蛋白之一（1）。NHERF1是首个识别的Na+/H+交换泵亚型3(NHE3)的腺苷环化酶相关的抑制所必需的协同作用因子之一（2）。NHERF1是一个多功能接头蛋白，它能通过PDZ结构域与受体和离子转运蛋白相互作用，能通过羧基末端与包括merlin在内的ERM家族蛋白质相互作用（2,3）。NHERF1在乳腺癌中发挥重要作用。雌激素被发现可以在雌激素受体阳性的乳腺癌细胞中诱导NHERF1（2,3）。另外，NHERF1还被发现可以结合PDGFR，其在乳腺癌中被激活。NHERF1被发现可以促进PTEN、NHERF1、PDGFR三元复合物的形成。NHERF1的功能主要是募集PTEN到PDGFR，抑制正常细胞的PI3K/Akt信号通路，这一作用在肿瘤中被打乱（4）。NHRF1也能结合囊性纤维化跨膜转导调节因子（CFTR），CFTR作为离子通道，变异可导致囊性纤维化（5）。NHERF1其它推测的功能包括睾丸分化、胞内回收、膜定位、蛋白质分拣和转运（6）。

1. Donowitz, M. et al. (2005) J Physiol 567, 3-11.
2. Voltz, J.W. et al. (2001) Oncogene 20, 6309-14.
3. Stemmer-Rachamimov, A.O. et al. (2001) Am J Pathol 158, 57-62.
4. Takahashi, Y. et al. (2006) EMBO J 25, 910-20.
5. Wheeler, D. et al. (2007) J Biol Chem 282, 25076-87.
6. Weinman, E.J. et al. (2000) Am J Physiol Renal Physiol 279, F393-9.

原厂资料：

Specificity / Sensitivity

NHERF1 (A140) Antibody detects endogenous levels of total NHERF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Ala140 of human NHERF1. Antibodies are purified by peptide affinity chromatography.

Background

Na+/H+ exchanger regulatory factor (NHERF1 or EBP-50) is one of several related PDZ domain-containing proteins (1). NHERF1 was first identified as a necessary cofactor for cyclic AMP-associated inhibition of Na+/ H+ exchanger isoform 3 (NHE3) (2). NHERF1 is a multifunctional adaptor protein that interacts with receptors and ion transporters via its PDZ domains and with the ERM family of proteins including merlin via its carboxy-terminus (2,3). NHERF1 may play an important role in breast cancer. Estrogen has been found to induce NHERF1 in estrogen receptor-positive breast cancer cells (2,3). Furthermore, NHERF1 has been shown to bind to PDGFR, which is activated in breast carcinomas. NHERF1 has been found to promote the formation of a ternary complex containing PTEN, NHERF1 and PDGFR. Thereby NHERF1 may function to recruit PTEN to PDGFR to inhibit the activation of the PI3K/Akt signaling in normal cells, and this mechanism may be disrupted in cancer (4). NHRF1 also binds to the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as an ion channel and has disease-causing mutations in Cystic fibrosis (5). Other proposed functions of NHERF1 include testicular differentiation, endosomal recycling, membrane targeting, protein sorting and trafficking (6).

1. Donowitz, M. et al. (2005) J Physiol 567, 3-11.
2. Voltz, J.W. et al. (2001) Oncogene 20, 6309-14.
3. Stemmer-Rachamimov, A.O. et al. (2001) Am J Pathol 158, 57-62.
4. Takahashi, Y. et al. (2006) EMBO J 25, 910-20.
5. Wheeler, D. et al. (2007) J Biol Chem 282, 25076-87.
6. Weinman, E.J. et al. (2000) Am J Physiol Renal Physiol 279, F393-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.