Phospho-Tyrosine Hydroxylase (Ser31) Antibody

• 产品编号：CST-3370S      品牌：CST       原厂货号：3370S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Tyrosine Hydroxylase（(Ser31) Antibody 兔多抗识别内源性的Ser31磷酸化的酪氨酸羟化酶。该多克隆抗体由合成的小鼠酪氨酸羟化酶Ser31附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。酪氨酸羟化酶（TH）催化神经递质多巴胺和其他儿茶酚胺类物质合成的限速步骤。TH以四聚体形式发挥功能，每个亚基都有一个调节域和一个催化域，其存在几个不同的异构体（1,2）。该酶在胚胎发育中必需，TH敲除的小鼠在出生前死亡（3）。TH的转录、翻译和翻译后修饰水平均能调节其活性。其氨基末端调节域包含三个丝氨酸残基：Ser9, Ser31和Ser40。PKA对Ser40的磷酸化正向调节TH的催化活性（4-6）。CDK5对Ser31的磷酸化也能够通过稳定TH蛋白水平来增加TH的催化活性（7-9）。

1. Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62.
2. Bodeau-Péan, S. et al. (1999) J Biol Chem 274, 3469-75.
3. Kobayashi, K. et al. (1995) J Biol Chem 270, 27235-43.
4. Lew, J.Y. et al. (1999) Mol Pharmacol 55, 202-9.
5. Vié, A. et al. (1999) J Biol Chem 274, 16788-95.
6. Lindgren, N. et al. (2000) J Neurochem 74, 2470-7.
7. Moy, L.Y. and Tsai, L.H. (2004) J Biol Chem 279, 54487-93.
8. Lehmann, I.T. et al. (2006) J Biol Chem 281, 17644-51.
9. Saraf, A. et al. (2007) J Biol Chem 282, 573-80.

原厂资料：

Specificity / Sensitivity

Phospho-Tyrosine Hydroxylase (Ser31) Antibody detects endogenous levels of tyrosine hydroxylase only when phosphorylated at Ser31.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Ser31 of mouse tyrosine hydroxylase. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9).

1. Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62.
2. Bodeau-Péan, S. et al. (1999) J Biol Chem 274, 3469-75.
3. Kobayashi, K. et al. (1995) J Biol Chem 270, 27235-43.
4. Lew, J.Y. et al. (1999) Mol Pharmacol 55, 202-9.
5. Vié, A. et al. (1999) J Biol Chem 274, 16788-95.
6. Lindgren, N. et al. (2000) J Neurochem 74, 2470-7.
7. Moy, L.Y. and Tsai, L.H. (2004) J Biol Chem 279, 54487-93.
8. Lehmann, I.T. et al. (2006) J Biol Chem 281, 17644-51.
9. Saraf, A. et al. (2007) J Biol Chem 282, 573-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.