PI3 Kinase Class III (D4E2) Rabbit mAb

• 产品编号：CST-3358S      品牌：CST       原厂货号：3358S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PI3 Kinase Class III (D4E2) Rabbit mAb兔单抗能够检测内源性的PI3K class III总蛋白。该单克隆抗体是由合成的人源的针对PI3K class III蛋白氨基末端序列的肽段免疫动物而生产的。3种不同类型的3-磷酸肌醇激酶（PI3K）已近被鉴别。和其他的PI3Ks不同，PI3K class III 能够催化磷脂酰肌醇 在D3位点的磷酸化，产生3-磷酸磷脂酰肌醇(PI(3)P) (1)。 PI3K class III 是Vps34,的哺乳动物同系物，最先是在酵母中发现。 PI3K class III 能够和调节性亚基p150相互作用，而p150是Vps15的哺乳动物同系物，能够通过十四（烷）酰化调节细胞膜的活动（2,3）。 PI3K class III能够通过FYVE 或者PX基团招募不同的蛋白到细胞膜上，从而调节膜泡运输和蛋白分选（4））。更主要的是， PI3K class III 能够调节细胞的自我吞噬， trimeric G蛋白的信号传导和mTOR营养敏感通路（5）。

1. Odorizzi, G. et al. (2000) Trends Biochem Sci 25, 229-35.
2. Panaretou, C. et al. (1997) J Biol Chem 272, 2477-85.
3. Kihara, A. et al. (2001) J Cell Biol 152, 519-30.
4. Corvera, S. (2001) Traffic 2, 859-66.
5. Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.

原厂资料：

Specificity / Sensitivity

PI3 Kinase Class III (D4E2) Rabbit mAb detects endogenous levels of total PI3K class III protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the amino-terminal sequence of human PI3K class III.

Background

Three distinct types of phosphoinositide 3-kinases (PI3K) have been characterized. Unlike other PI3Ks, PI3K class III catalyzes the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate (PIP3) (1). PI3K class III is the mammalian homolog of Vps34, first identified in yeast. PI3K class III interacts with the regular subunit p150, the mammalian homolog of Vps15, which regulates cellular membrane association through myristoylation (2,3). PIP3 recruits several proteins with FYVE or PX domains to membranes regulating vesicular transport and protein sorting (4). Moreover, PI3K class III has been shown to regulate autophagy, trimeric G-protein signaling, and the mTOR nutrient-sensing pathway (5).

1. Odorizzi, G. et al. (2000) Trends Biochem Sci 25, 229-35.
2. Panaretou, C. et al. (1997) J Biol Chem 272, 2477-85.
3. Kihara, A. et al. (2001) J Cell Biol 152, 519-30.
4. Corvera, S. (2001) Traffic 2, 859-66.
5. Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.