CD79A Antibody

• 产品编号：CST-3351S      品牌：CST       原厂货号：3351S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CD79A 抗体能够检测内源CD79A蛋白总体水平。此多克隆抗体是通过合成人源对应的CD79A Asn217位点周围的肽段来免疫动物而获得。抗体是通过多肽亲和层析纯化。B细胞上的抗原受体包含了异源二聚体信号成员，此二聚体由CD79A 和CD79B组成,也分别称作是Ig α和Ig β(1,2)。此受体复合体的存在对B细胞的发育和功能有着重要的意义(3)。抗原结合后，这两个蛋白和B细胞相关的受体一起起始胞内的信号转导T(4,5)。一个免疫受体酪氨酸相关的激活区域(ITAM)存在于CD79A胞内区域并对其功能发挥了重大的作用(6)。抗原结合，然后形成CD79A 和 CD79B异源二聚体，最后激活受体相关的激酶(7)。CD79A是B细胞系成淋巴细胞白血病的一个特征分子(8); 在有些慢性B淋巴细胞白血病中，对应mb-1基因的突变对异常低水平的功能性B细胞受体有重要的作用(9)。

1. van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
2. Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
3. Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
4. Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
5. Mason, D.Y. et al. (1995) Blood 86, 1453-9.
6. Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
7. Pike, K.A. et al. (2004) J Immunol 172, 2210-8.
8. Astsaturov, I.A. et al. (1996) Leukemia 10, 769-73.
9. Vuillier, F. et al. (2005) Blood 105, 2933-40.

原厂资料：

Specificity / Sensitivity

CD79A Antibody detects endogenous levels of total CD79A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn217 of human CD79A. Antibodies are purified by peptide affinity chromatography.

Background

Antigen receptors found on the surface of B-cells contain a hetrodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B-cell development and function (3). Together these two proteins and the associated B cell receptor initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) is found in the CD79A intracellular region appears to be is important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). CD79A is a marker for B-lineage lymphoblastic leukemia (8); mutations in the corresponding mb-1 gene are responsible for abnormally low levels of functional B cell receptors in some cases of chronic B-cell lymphocytic leukemia (9).

1. van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
2. Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
3. Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
4. Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
5. Mason, D.Y. et al. (1995) Blood 86, 1453-9.
6. Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
7. Pike, K.A. et al. (2004) J Immunol 172, 2210-8.
8. Astsaturov, I.A. et al. (1996) Leukemia 10, 769-73.
9. Vuillier, F. et al. (2005) Blood 105, 2933-40.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.