SPHK1 Antibody

• 产品编号：CST-3297S      品牌：CST       原厂货号：3297S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PHK1抗体识别内源性的全部SPHK1蛋白。该多克隆抗体用与人类SPHK1蛋白氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白亲和层析纯化而得。鞘氨醇激酶（SPHKs）催化鞘氨醇磷酸化形成鞘氨醇-1-磷酸盐（S1P），一种在细胞内外发挥功能的脂质介导分子。SPHKs，连同其它鞘脂样代谢酶，调节脂质介质，神经酰胺，鞘氨醇和S1P的平衡（1-4）。两个不同的SPHK亚型，SPHK1和SPHK2已被克隆和鉴定（5,6）。SPHK1和SPHK2高度保守，但表达相异（7,8）。SPHKs可被G-蛋白偶联受体、受体酪氨酸激酶、免疫球蛋白受体、细胞因子和其他刺激激活（9-12）。SPHK1和SPHK2的具体调控分子机制是复杂的，目前只有部分的理解。

1. Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26.
2. Xia, P. et al. (2000) Curr Biol 10, 1527-30.
3. Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903.
4. Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8.
5. Kohama, T. et al. (1998) J Biol Chem 273, 23722-8.
6. Liu, H. et al. (2000) J Biol Chem 275, 19513-20.
7. Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511.
8. Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407.
9. Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28.
10. Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34.
11. Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8.
12. Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39.

原厂资料：

Specificity / Sensitivity

SPHK1 Antibody detects endogenous levels of total SPHK1 protein.S

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human SPHK1. Antibodies are purified by peptide affinity chromatography.

Background

Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Together with other sphingolipid metobolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5,6). SPHK1 and SPHK2 are highly conserved and diverselly expressed (7,8). The SPHKs are activated by G-protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood.

1. Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26.
2. Xia, P. et al. (2000) Curr Biol 10, 1527-30.
3. Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903.
4. Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8.
5. Kohama, T. et al. (1998) J Biol Chem 273, 23722-8.
6. Liu, H. et al. (2000) J Biol Chem 275, 19513-20.
7. Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511.
8. Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407.
9. Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28.
10. Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34.
11. Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8.
12. Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.