Phospho-Pyk2 (Tyr402) Antibody

• 产品编号：CST-3291S      品牌：CST       原厂货号：3291S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Pyk2 (Tyr402) Antibody兔多抗能检测内源性Tyr402位点磷酸化的Pyk2蛋白水平。该抗体可能与其他含有磷酸化酪氨酸的蛋白发生交叉反应。该多克隆抗体是采用合成的与人源 Pyk2蛋白Tyr402位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。蛋白酪氨酸激酶Pyk2, 也被称为CAKβ, RAFTK和CADTK，是一种非受体酪氨酸激酶，在结构上与粘着斑激酶(FAK)相关(1-4)。Pyk2主要表达于来源于造血细胞系的细胞中和中枢神经系统中。Pyk2是G蛋白偶联受体和MAPK信号转导途径的一个信号转导调节子。它在细胞伸展和迁移过程中起着重要的作用(5-7)。TCR的结合能够使Pyk2的酪氨酸磷酸化，从而被激活(8,9)。Pyk2蛋白Tyr402位点的磷酸化是Pyk2蛋白中其他酪氨酸的磷酸化所必须的，并且在T细胞活化过程中为Fyn SH2提供结合位点(10)。

1. Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
2. Lev, S. et al. (1995) Nature 376, 737-745.
3. Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
4. Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
5. Duong, L. T. et al. (2001) J. Biol. Chem. 276, 7484-7492.
6. Watson, J. M. et al. (2001) J. Biol. Chem. 276, 3536-3542.
7. Tang, H. et al. (2002) J. Biol. Chem. 277, 5441-5447.
8. Berg, N.N. and Ostergaard, H.L. (1997) J. Immunol. 159, 1753-1757.
9. Ganju, R. K. et al. (1997) J. Exp. Med. 185, 1055-1063.
10. Katagiri, T. et al. (2000) J. Biol. Chem. 275, 19645-19652.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Phospho-Pyk2 (Tyr402) Antibody detects endogenous levels of Pyk2 only when phosphorylated at tyrosine 402. This antibody may cross-react with other phospho-tyrosine containing proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr402 of human Pyk2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein tyrosine kinase Pyk2, also called CAKβ, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR (8,9). Phosphorylated Tyr402 of Pyk2 is required for the phosphorylation of other tyrosines on Pyk2 and provides a binding site for Fyn SH2 in the T-cell activation (10).

1. Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
2. Lev, S. et al. (1995) Nature 376, 737-745.
3. Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
4. Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
5. Duong, L. T. et al. (2001) J. Biol. Chem. 276, 7484-7492.
6. Watson, J. M. et al. (2001) J. Biol. Chem. 276, 3536-3542.
7. Tang, H. et al. (2002) J. Biol. Chem. 277, 5441-5447.
8. Berg, N.N. and Ostergaard, H.L. (1997) J. Immunol. 159, 1753-1757.
9. Ganju, R. K. et al. (1997) J. Exp. Med. 185, 1055-1063.
10. Katagiri, T. et al. (2000) J. Biol. Chem. 275, 19645-19652.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody