Phospho-SGK (Ser78) Antibody

• 产品编号：CST-3271S      品牌：Cell Signaling Technology       原厂货号：3271S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-SGK (Ser78) Antibody 能够检测转染的Ser78位点磷酸化的SGK1蛋白，不能检测其异构体SGK2或SGK3。该多克隆抗体是由合成的人源的针对SGK蛋白Ser78位点的磷酸化肽段免疫动物而生产的。抗体由protein A和肽亲和层析技术纯化。血清和糖皮质激素诱导激酶（SGK）是一个丝氨酸/苏氨酸激酶，与Akt紧密联系（1）。SGK可以被多种刺激包括血清，糖皮质激素，卵泡刺激素，渗透压冲击和盐皮质激素迅速诱导。SGK可以被肝细胞生长因子刺激的PI3K依赖的通路和整合素介导的PI3K非依赖的通路激活（2,3）。SGK的诱导和激活参与激活细胞抗凋亡和细胞周期调控（4-6）。SGK在钾，钠，氯离子通道激活中也起重要作用，表明其参与调控细胞的存活，神经元的兴奋，肾钠排泄等过程（2）。SGK被泛素化和蛋白酶体降解等过程负向调控（7）。除了细胞膜通道和载体功能，SGK1也可以调控多种靶蛋白，例如GSK-3, NEDD4, iNOS, AMPAR, PSD95, Tau, NF-κB, CREB, 和 FKHR-L1 (8,9)。MAP激酶家族成员BMK1与SGK相互作用并通过磷酸化修饰其Ser78位点激活SGK（6）。

1. Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.
2. Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.
3. Park, J. et al. (1999) EMBO J 18, 3024-33.
4. Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.
5. Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.
6. Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.
7. Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-SGK (Ser78) Antibody detects transfected levels of SGK1 only when phosphorylated at serine 78. It will not detect isoforms SGK2 or SGK3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser78 of human SGK. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7). The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78 (6).

1. Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.
2. Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.
3. Park, J. et al. (1999) EMBO J 18, 3024-33.
4. Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.
5. Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.
6. Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.
7. Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.