CLK3 Antibody

• 产品编号：CST-3256S      品牌：CST       原厂货号：3256S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CLK3 Antibody检测内源性CLK3蛋白的全长和截短形式额水平。通过人工合成CLK3蛋白His51位点周围序列相应的多肽片段去免疫动物从而制备抗体。 通过蛋白A和多肽亲和层析纯化抗体。cdc2-like kinase (CLK)家族至少包含四种高度保守的异构体: CLK1、CLK2、CLK3和CLK4 (1,2)。CLKs蛋白家族是双特异性蛋白激酶，即能在丝氨酸、苏氨酸和酪氨酸位点发生自磷酸化，又能使外源性蛋白的丝氨酸和苏氨酸位点发生磷酸化(2)。CLK家族成员以具有催化活性的全长形式和无活性的选择性剪切的截短形式存在(1)。CLKs蛋白家族可磷酸化一类高度磷酸化的蛋白，这类蛋白称为丝氨酸和精氨酸富集(SR)蛋白(3-5)。SR蛋白是一类剪接因子，调节剪接体的装配。而细胞核内RNA剪切过程发生在剪接体中。SR蛋白也涉及到剪接位点的选择。因此，CLKs可能在RNA剪接的调节中起到重要作用。

1. Hanes, J. et al. (1994) J. Mol. Biol. 244, 665-672.
2. Nayler, O. et al. (1997) Biochem. J. 326, 693-700.
3. Colwill, K. et al. (1996) EMBO J. 15, 265-275.
4. Prasad, J. and Manley, J.L. (2003) Mol. Cell Biol. 23, 4139-4149.
5. Muraki, M. et al. (2004) J. Biol. Chem. 279, 24246-24254.
6. Menegay, H. et al. (1999) Exp. Cell Res. 253, 463-473.
7. Becker, W. et al. (1996) Biochim. Biophys. Acta 1312, 63-67.
8. Duncan, P.I. et al. (1998) Exp. Cell Res. 241, 300-308.

原厂资料：

Specificity / Sensitivity

CLK3 Antibody detects endogenous levels of full-length and truncated forms of CLK3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His51 of CLK3. Antibodies were purified by protein A and peptide affinity chromatography.

Background

The cdc2-like kinase (CLK) family contains at least four highly conserved isoforms: CLK1, CLK2, CLK3 and CLK4 (1,2). CLKs are dual specificity kinases that autophosphorylate on serine, threonine and tyrosine residues and phosphorylate exogenous substrates on serine and threonine residues (2). CLK family members exist as both a full-length catalytically active form and an alternatively-spliced, inactive truncated form (1). A family of highly phosphorylated proteins, called serine and arginine rich (SR) proteins, are phosphorylated by CLKs (3-5). SR proteins are splicing factors that regulate the assembly of the spliceosome, a macromolecular complex where RNA splicing occurs in the nucleus. They are also involved in the selection of splice sites. Thus, CLKs may play important roles in regulating RNA splicing.

1. Hanes, J. et al. (1994) J. Mol. Biol. 244, 665-672.
2. Nayler, O. et al. (1997) Biochem. J. 326, 693-700.
3. Colwill, K. et al. (1996) EMBO J. 15, 265-275.
4. Prasad, J. and Manley, J.L. (2003) Mol. Cell Biol. 23, 4139-4149.
5. Muraki, M. et al. (2004) J. Biol. Chem. 279, 24246-24254.
6. Menegay, H. et al. (1999) Exp. Cell Res. 253, 463-473.
7. Becker, W. et al. (1996) Biochim. Biophys. Acta 1312, 63-67.
8. Duncan, P.I. et al. (1998) Exp. Cell Res. 241, 300-308.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.