TTK Antibody

• 产品编号：CST-3255S      品牌：CST       原厂货号：3255S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TTK Antibody能够检测内源性TTK总蛋白。TTK又称为PYT(磷酸化酪氨酸特异性苏氨酸激酶)，是出芽酵母和鼠ESK (胚胎肿瘤STY激酶)蛋白对应的人MSP1(单极纺锤体蛋白激酶 1)同源蛋白。多克隆抗体是由合成的人源的针对TTK蛋白谷氨酸（137位）的肽段免疫动物，采用亲和层析技术纯化生产的。 TTK（MPS1，PYT）是一种细胞周期调控的双特异性激酶，存在于快速增殖的组织和细胞系（1-3）。TTK集中在着丝粒和中心体，是有丝分裂纺锤体检验点和中心体复制的必不可少的成分(4-6)。有丝分裂检查点抑制后期的进入，直到所有的染色体进入纺锤体；抑制这一过程将导致基因组不稳定和肿瘤的发生。TTK介导的BLM第144位丝氨酸磷酸化能够保持有丝分裂染色体的稳定性(7)。TTK的小分子抑制剂能够阻遏纺锤体检验点反应，由此使TTK成为一个潜在的治疗靶标(8,9)。TTK也参与DNA损伤反应，通过直接磷酸化并激活细胞周期检查点激酶Chk2的第68位苏氨酸。Chk2的两个靶磷酸化靶标分别是细胞周期磷酸酶cdc25和转录因子p53。Cdc25磷酸酶的失活将导致周期蛋白B的累积和伴随DNA损伤的细胞周期捕获。活化的Chk2磷酸化p53能够稳定转录因子并促进细胞周期捕获和凋亡来应答DNA损伤(10)。

1. Mills, G.B. et al. (1992) J. Biol. Chem. 267, 16000-16006.
2. Stucke, V.M. et al. (2002) EMBO J. 21, 1723-1732.
3. Lindberg, R.A. et al. (1993) Oncogene 8, 351-359.
4. Fisk, H.A. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 14875-14880.
5. Dou, Z. et al. (2003) Cell Res. 13, 443-449.
6. Abrieu, A. et al. (2001) Cell 106, 83-93.
7. Leng, M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 11485-11490.
8. Schmidt, M. et al. (2005) EMBO Rep. 6, 866-872.
9. Dorer, R.K. et al. (2005) Curr. Biol. 15, 1070-1076.
10. Wei, J.H. et al. (2005) J. Biol. Chem. 280, 7748-7757.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

TTK Antibody detects endogenous levels of total TTK protein. TTK, also known as PYT (Phosphotyrosine Picked Threonine Kinase), is the human homolog of MSP1 (Monopolar Spindle 1) in budding yeast and murine ESK (Embryonal Carcinoma STY Kinase).T

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu137 of human TTK. Antibodies were purified by affinity chromatography.

Background

TTK (Mps1, PYT) is a cell cycle regulated dual specificity kinase present in rapidly proliferating tissues and cell lines (1-3). TTK localizes to kinetochores and centromeres and is an essential component of the mitotic spindle checkpoint as well as centrosome duplication (4-6). The mitotic checkpoint inhibits entry into anaphase until all chromosomes are attached to the spindle; inhibition of this process leads to genomic instability and tumorigenesis. Phosphorylation of the BLM helicase at Ser144 by TTK maintains chromosome stability during mitosis (7). Small molecule inhibitors of TTK can block the spindle checkpoint response, thereby making TTK a potential therapeutic target (8,9). 
 TTK also participates in the DNA damage response by directly phosphorylating and activating the cell cycle checkpoint kinase Chk2 at Thr68. Two targets phosphorylated by Chk2 are the cell cycle phosphatase cdc25 and the transcription factor p53. Inactivation of cdc25 phosphatase results in the accumulation of inactive cyclin B and cell cycle arrest following DNA damage. Phosphorylation of p53 by active Chk2 stabilizes the transcription factor and promotes cell cycle arrest and apoptosis in response to DNA damage (10). 

1. Mills, G.B. et al. (1992) J. Biol. Chem. 267, 16000-16006.
2. Stucke, V.M. et al. (2002) EMBO J. 21, 1723-1732.
3. Lindberg, R.A. et al. (1993) Oncogene 8, 351-359.
4. Fisk, H.A. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 14875-14880.
5. Dou, Z. et al. (2003) Cell Res. 13, 443-449.
6. Abrieu, A. et al. (2001) Cell 106, 83-93.
7. Leng, M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 11485-11490.
8. Schmidt, M. et al. (2005) EMBO Rep. 6, 866-872.
9. Dorer, R.K. et al. (2005) Curr. Biol. 15, 1070-1076.
10. Wei, J.H. et al. (2005) J. Biol. Chem. 280, 7748-7757.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody