Phospho-Caveolin-1 (Tyr14) Antibody

• 产品编号：CST-3251P      品牌：Cell Signaling Technology       原厂货号：3251P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Caveolin-1 (Tyr14) Antibody检测仅在tyrosine 14位点磷酸化的内源性caveolin-1蛋白。该抗体不能与caveolin-2、3或caveolin-1beta（caveolin-1蛋白短的亚型）发生交叉反应。通过人工合成人源caveolin-1蛋白Tyr14位点周围相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。caveolins蛋白分子量是21-24 kDa，其富含胆固醇/鞘磷脂膜的质膜微区caveolae的主要结构成分。caveolin蛋白家族三个成员（caveolin-1、-2和-3）在不同组织分布已经被鉴定。Caveolins蛋白形成异源二聚体和同源二聚体，其能与胆固醇和其它脂质相互作用 (1)。Caveolins蛋白涉及不同生物功能，包括囊泡运输、胆固醇体内平衡、细胞粘附和凋亡，并且也涉及到神经退行性疾病 (2)。Caveolins蛋白也与多种信号分子相互作用，例如Gα亚型、酪氨酸激酶受体、PKCs、Src家族酪氨酸激酶和eNOS(1,2)。有研究认为caveolins 蛋白作为对信号转导的整合的支架蛋白 。在 Tyr14位点磷酸化对caveolin蛋白与SH2或PTB区域包含受体蛋白例如GRB7的联系有本质作用(3-5)。在Ser80 位点的磷酸化调节caveolin蛋白结合ER膜，并且进入分泌通路(6)。

1. Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
2. Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304.
3. Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
4. Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
5. Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75.
6. Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

Phospho-Caveolin-1 (Tyr14) Antibody detects endogenous levels of caveolin-1 only when phosphorylated at tyrosine 14. The antibody does not cross-react with caveolin-2, -3 or caveolin-1beta, the short isoform of caveolin-1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr14 of human caveolin-1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2 and -3) have been identified with different tissue distributions. Caveolins form hetero- and homooligomers, which interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins also interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6). 

1. Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
2. Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304.
3. Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
4. Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
5. Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75.
6. Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.