Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb

• 产品编号：CST-3233S      品牌：CST       原厂货号：3233S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb兔单抗能够检测内源性的酪氨酸（627位点）磷酸化的Gab1蛋白。抗体也可以与磷酸化的Gab2及磷酸化的Gab3或者活化的受体酪氨酸激酶(RTKs)发生交叉反应。该单克隆抗体是由合成与人源Gab1蛋白Tyr627的周围序列相对应的磷酸化肽段免疫动物而生产的。Grb相关结合因子（Gab）家族是一类由多种受体酪氨酸激酶（RTKs），如EGFR，HGFR，胰岛素受体，细胞因子受体和B细胞抗原受体招募的接头蛋白。RTKs受到它们的同源配体刺激后，Gab被招募到细胞膜上，接着其被磷酸化，并起到支架的作用（1-4）。Gab1蛋白存在多个酪氨酸磷酸化位点（5）。酪氨酸（472位点）磷酸化可以调节其与PI3K激酶p85的结合（6,7）。Gab1酪氨酸（307，373和407位点）的磷酸化可以调节Gab1与PLCγ的互作（8）。酪氨酸（627和659位点）磷酸化是Gab1结合并活化蛋白酪氨酸磷酸酶SHP2所必需的（6,9）。

1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
4. Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
7. Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
8. Gual, P. et al. (2000) Oncogene 19, 1509-18.
9. Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb detects endogenous levels of Gab1 only when phosphorylated at Tyr627. The antibody may cross-react with phosphorylated Gab2 or Gab3, or with activated receptor tyrosine kinases (RTKs).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr627 of human Gab1.

Background

The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).

1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
4. Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
7. Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
8. Gual, P. et al. (2000) Oncogene 19, 1509-18.
9. Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody