Aldolase A Antibody

• 产品编号：CST-3188S      品牌：CST       原厂货号：3188S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Aldolase抗体识别内源性的果糖二磷酸醛缩酶总蛋白水平（包括醛缩酶A，B和C）。该多克隆抗体用与人类果糖二磷酸醛缩酶氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。Aldolase(醛缩酶)（果糖二磷酸醛缩酶）是一种糖酵解酶，催化1，6- 二磷酸果糖转变为3磷酸甘油醛。这种无处不在的酶有三个不同的同工酶：醛缩酶A，醛缩酶B和醛缩酶C。研究表明醛缩酶A的定量分析对肝癌有潜在的诊断价值（1）。此外，醛缩酶B基因表达水平的变化也在某些原发肿瘤病人中被观察到（2,3）。

1. Castaldo, G. et al. (2000) Clin. Chem. 46, 901-906.
2. Kinoshita, M. and Miyata, M. (2002) Hepatology 36, 433-438.
3. Song, H. et al. (2004) World J. Gastroenterol 10, 509-513.

原厂资料：

Specificity / Sensitivity

Aldolase Antibody detects endogenous levels of total fructose bisphosphate aldolase (including aldolases A, B and C) protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of humanfructose bisphosphate aldolase. Antibodies are purified by protein A and peptide affinity chromatography.

 Background

Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B and aldolase C. Studies suggest the potential diagnostic value of quantitive anlysis of aldolase A for hepatocarcinoma (1). Furthermore, the change of aldolase B gene expression levels has also been observed in certain patients with this primary tumor (2,3).

1. Castaldo, G. et al. (2000) Clin. Chem. 46, 901-906.
2. Kinoshita, M. and Miyata, M. (2002) Hepatology 36, 433-438.
3. Song, H. et al. (2004) World J. Gastroenterol 10, 509-513.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.