FoxA2/HNF3β Antibody

• 产品编号：CST-3143S      品牌：CST       原厂货号：3143S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FoxA2/HNF3β抗体识别内源性的FoxA2/HNF3β总蛋白水平。该多克隆抗体用与人类FoxA2/HNF3β氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和肽亲和层析纯化而得。叉头框（Forkhead box）蛋白A2（FoxA2，也被称作肝细胞核因子3β或HNF3β）是一个转录因子，在肝细胞功能中扮演重要角色（1）。FoxA2/HNF3β是空腹时肝生糖基因表达所必需的（1）。与协同因子PGC-1β一起，FoxA2/HNF3β可以刺激脂肪酸β氧化相关基因的表达，增强脂肪酸的代谢（2）。FoxA2/HNF3β，与PGC-1β一起，可以激活微粒体甘油三酯转运蛋白（MTP）的表达，促进VLDL分泌（2）。另外，除了其在代谢综合症中发挥的作用，FoxA2/HNF3β也对小鼠胚胎内胚层和中线结构的发育至关重要（3-5）。

1. Zhang, L. et al. (2005) Cell Metab 2, 141-8.
2. Wolfrum, C. and Stoffel, M. (2006) Cell Metab 3, 99-110.
3. Levinson-Dushnik, M. and Benvenisty, N. (1997) Mol Cell Biol 17, 3817-22.
4. Weinstein, D.C. et al. (1994) Cell 78, 575-88.
5. Ang, S.L. and Rossant, J. (1994) Cell 78, 561-74.

原厂资料：

Specificity / Sensitivity

FoxA2/HNF3β Antibody detects endogenous levels of total FoxA2/HNF3β protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human FoxA2/HNF3β. Antibodies are purified by Protein A and peptide affinity chromatography.

Background

Forkhead box protein A2 (FoxA2, also known as hepatocyte nuclear factor 3β or HNF3β) is a transcription factor that plays an important role in hepatocyte function (1). FoxA2/HNF3β is required for the activation of hepatic gluconeogenic gene expression during fasting (1). Together with the PGC-1β coactivator, FoxA2/HNF3β stimulates the expression of genes involved in fatty acid β-oxidation and therefore increases fatty acid metabolism (2). FoxA2/HNF3β, along with PGC-1β, also activates the expression of microsomal triacylglycerol transfer protein (MTP) and promotes VLDL secretion (2). In addition to its roles in metabolic syndromes, FoxA2/HNF3β is essential for development of the endoderm and midline structures in mouse embryos (3-5).

1. Zhang, L. et al. (2005) Cell Metab 2, 141-8.
2. Wolfrum, C. and Stoffel, M. (2006) Cell Metab 3, 99-110.
3. Levinson-Dushnik, M. and Benvenisty, N. (1997) Mol Cell Biol 17, 3817-22.
4. Weinstein, D.C. et al. (1994) Cell 78, 575-88.
5. Ang, S.L. and Rossant, J. (1994) Cell 78, 561-74.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.